Ultraviolet irradiation of platelet concentrates: feasibility in transfusion practice
Ultraviolet irradiation of platelet concentrates: feasibility in transfusion practice
复制标题
浓缩血小板的紫外线照射：输血实践的可行性
DOI：
10.1046/j.1537-2995.1990.30590296370.x
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
M. Samama
中科院分区：
文献类型：
--
作者：
G. Andreu;C. Boccaccio;C. Lecrubier;J. Frétault;J. Coursaget;J. Leguen;M. Oleggini;J. Fournel;M. Samama
紫外线 (UV)-B 照射会消除混合淋巴细胞培养物 (MLC) 中的淋巴细胞功能（反应和刺激的能力）。这种效应可能在预防或减少输血诱导的针对 HLA I 类抗原的同种免疫中具有实际应用。为了研究这一点，用细胞分离器获得血小板浓缩物（PC），悬浮在自体血浆中，最终体积为 400 mL，然后转移到大（22 x 30 cm）细胞培养袋中。这种塑料在 310 nm 处表现出良好的 UV-B 射线透过率 (54%)。将PC放置在两块石英板之间（照射面积=25×37cm），两侧同时照射。自动监测传递到塑料袋表面的能量。每平方厘米 0.75 焦耳的能量（照射时间少于 3 分钟）完全消除了反应能力（MLC 和植物血凝素）和刺激同种异体淋巴细胞的能力。照射期间的温度升高可以忽略不计。如果 UV-B 能量低于 3 J/cm2，血小板聚集（胶原蛋白、肾上腺素、ADP、花生四烯酸、瑞斯托菌素）不会受到损害。在四名志愿者中研究了自体 111in 标记血小板的恢复和存活；经过 UV-B 处理 (1.5 J/cm2) 的血小板和未经处理的血小板之间没有发现差异。这些结果表明，使用 UV-B 照射的 PC 来预防 HLA 同种免疫的大规模临床试验是可行的。
Ultraviolet (UV)‐B irradiation abolishes lymphocyte functions (the ability to respond and to stimulate) in mixed lymphocyte culture (MLC). This effect may have practical application in the prevention or reduction of transfusion‐induced alloimmunization against HLA class I antigens. To study this, platelet concentrates (PCs) were obtained with a cell separator, suspended in autologous plasma in a final volume of 400 mL, and transferred into a large (22 times 30 cm) cell culture bag. This plastic showed a good transmittance of UV‐B rays at 310 nm (54%). PCs were placed between two quartz plates (surface of irradiation = 25 times 37 cm), and the two sides were irradiated simultaneously. Energy delivered to the surface of the plastic bag was automatically monitored. The ability to respond (in MLC and to phytohemagglutinin) and to stimulate allogeneic lymphocytes was completely abolished with energy of 0.75 J per cm2 (irradiation time less than 3 min). The temperature increase during irradiation was negligible. Platelet aggregation (collagen, adrenalin, ADP, arachidonic acid, ristocetin) was not impaired if UV‐B energy was below 3 J per cm2. Recovery and survival of autologous 111in‐labeled platelets were studied in four volunteers; no differences were found between UV‐B‐treated (1.5 J/cm2) platelets and untreated platelets. These results show that a large‐scale clinical trial using UV‐B‐irradiated PCs to prevent HLA alloimmunization is feasible.
功能性树突状细胞是犬骨髓移植受者输血诱导致敏所必需的。
影响因子：
20.3
作者：
Deeg,HJ;Aprile,J;Storb,R;Graham,TC;Hackman,R;Appelbaum,FR;Schuening,F
通讯作者：
Schuening,F