Ceramide formation during heat shock: A potential mediator of alpha B-crystallin transcription
Ceramide formation during heat shock: A potential mediator of alpha B-crystallin transcription
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DOI：
10.1073/pnas.92.26.12275
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发表时间：
1995-12-19
影响因子：
11.1
通讯作者：
Shayman, JA
中科院分区：
文献类型：
--
作者：
Chang, Y;Abe, A;Shayman, JA
神经酰胺已被鉴定为潜在的第二信使，其可在暴露于激素激动剂如1 α，25-二羟基维生素D-3、肿瘤坏死因子α或γ-干扰素后介导细胞分化和凋亡。神经酰胺产生后的次级细胞事件仍然不确定，我们报告说，在NIH WT-3 T3细胞中，神经酰胺诱导增强α B-晶体蛋白，一种小的热休克蛋白的基因转录。通过北方印迹和免疫印迹分析测量的α B-晶状体蛋白水平通过添加外源性短链神经酰胺N-乙酰鞘氨醇或通过抑制葡糖神经酰胺合酶增加内源性细胞内神经酰胺而增加。在密切相关的基因Hsp 25的表达中未观察到类似的效应。为了确定神经酰胺介导的基因转录是否是热休克反应的特征，在热休克细胞中测量细胞神经酰胺，并观察到细胞回到37 ℃后立即升高2倍。因此，热休克处理后形成的神经酰胺3 T3细胞可能介导的转录事件与细胞应激反应。
Ceramide has been identified as a potential second messenger that may mediate cell differentiation and apoptosis after exposure to hormonal agonists such as 1 alpha,25-dihydroxyvitamin D-3, tumor necrosis factor alpha, or gamma-interferon. The secondary cellular events that follow ceramide generation remain undefined, We report that in NIH WT-3T3 cells, ceramide induces an enhancement of gene transcription of alpha B-crystallin, a small heat shock protein. The levels of alpha B-crystallin, as measured by Northern blot and immunoblot analyses, were increased by the addition of an exogenous short-chain ceramide, N-acetylsphingosine, or by increasing endogenous intracellular ceramide by inhibition of glucosylceramide synthase, Similar effects were not seen in the expression of the closely related gene, Hsp25. To ascertain whether ceramide-mediated gene transcription was a feature of the heat shock response, cell ceramide was measured in heat shocked cells and observed to be elevated 2-fold immediately upon the return of cells to 37 degrees C. Thus ceramide formed after heat shock treatment of 3T3 cells may mediate the transcription events associated with the cell stress response.