Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages
Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages
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DOI：
10.1016/j.intimp.2004.05.008
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发表时间：
2004-08-01
影响因子：
5.6
通讯作者：
Yu, QH
中科院分区：
文献类型：
--
作者：
Wang, DY;Noda, Y;Yu, QH
本研究旨在探讨肝素钠对CaMK Ⅱ、CREB和ERK 1/2磷酸化及c-Fos表达的影响。在培养基中加入或不加入磺酸钠的情况下，体外培养大细胞。细胞培养后，裂解巨噬细胞并收集巨噬细胞的裂解物用于分析。采用Western-blotting方法研究这些信号元件的磷酸化或表达。本研究发现，经肝素钠处理后，巨噬细胞内CaMK II和CREB的磷酸化水平以及c-Fos蛋白的表达增加，而ERK 1/2的磷酸化水平不受其影响。(C)2004 Elsevier B. V.保留所有权利。
The purpose of this research is to investigate the effects of sodium houttuyfonate on the phosphorylation of CaMK II, CREB and ERK 1/2, and the expression of c-Fos. Macrophages were cultured in vitro with or without sodium houttuyfonate in the culture medium. After cell culture, macrophages were lysed and the lysate of the macrophages was collected for analysis. Western-blotting method was adopted to investigate the phosphorylation or the expression of these signal elements. It was found in this research that the phosphorylation levels of CaMK II and CREB and the expression of c-Fos protein in macrophages were increased by sodium houttuyfonate treatment; however, the phosphorylation level of ERK 1/2 was not affected by the treatment. (C) 2004 Elsevier B.V. All rights reserved.