Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25) Alters Resistance to Bacterial and Fungal Pathogens.
Overexpression of Rice Wall-Associated Kinase 25 (OsWAK25) Alters Resistance to Bacterial and Fungal Pathogens.
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DOI：
10.1371/journal.pone.0147310
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ronald PC
中科院分区：
文献类型：
--
作者：
Harkenrider M;Sharma R;De Vleesschauwer D;Tsao L;Zhang X;Chern M;Canlas P;Zuo S;Ronald PC
细胞壁相关激酶包括在植物生长和胁迫响应中起作用的受体样激酶的亚家族。先前的研究表明，水稻壁相关激酶OsWAK25与生物和非生物胁迫反应相关的多种蛋白质相互作用。在这里，我们表明，创伤和BTH处理诱导水稻OsWAK25转录本的表达。我们产生了OsWAK25过表达株系，并显示这些株系表现出病变模拟表型，并增强了水稻NH 1（NPR 1同源物1），OsPAL 2，PBZ 1和PR 10的表达。此外，这些品系显示出对半活体营养型病原体黄单胞菌致病型（Xanthomonasplantypv）的抗性。稻瘟病菌（Xoo）和稻瘟病菌（Magnaporthe）对坏死营养型真菌病原体立枯丝核菌（Rhizoctonia solani）和宫城旋孢菌（Cochliobolus miyabeanus）的敏感性增加。
Wall-associated kinases comprise a sub-family of receptor-like kinases that function in plant growth and stress responses. Previous studies have shown that the rice wall-associated kinase, OsWAK25, interacts with a diverse set of proteins associated with both biotic and abiotic stress responses. Here, we show that wounding and BTH treatments induce OsWAK25 transcript expression in rice. We generated OsWAK25 overexpression lines and show that these lines exhibit a lesion mimic phenotype and enhanced expression of rice NH1 (NPR1 homolog 1), OsPAL2, PBZ1 and PR10. Furthermore, these lines show resistance to the hemibiotrophic pathogens, Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae, yet display increased susceptibility to necrotrophic fungal pathogens, Rhizoctonia solani and Cochliobolus miyabeanus.