Relationship between serum reactive oxidative metabolite level and skin reaction in an irradiated rat model
Relationship between serum reactive oxidative metabolite level and skin reaction in an irradiated rat model
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DOI：
10.3109/10715762.2014.894637
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发表时间：
2014-05-01
影响因子：
3.3
通讯作者：
Nemoto, Kenji
中科院分区：
文献类型：
--
作者：
Nomiya, Takuma;Kaneko, Takashi;Nemoto, Kenji
目的。电离辐射会产生自由基和活性氧物种，从而在体内引起DNA损伤。本研究旨在探讨辐射后大鼠血清活性氧代谢物(ROM)水平与皮肤反应的关系。方法和材料。将雌性Wistar大鼠分为对照组、2Gy组和30Gy组，在急性期测定血清ROM值。另取雌性Wistar大鼠，随机分为0、30、50、70Gy4个剂量组，分别于照射前、照射后3、7、16、24、31、38天测定血清ROM值。每周2次按SRS(0~5)评分评定皮肤反应。结果。亚急性期50Gy组和70Gy组血清ROM值显著高于0Gy组和30Gy组[p=0.029，重复测量方差分析]。正如预期的那样，SRSS按0、30、50和70Gy组的顺序增加，并且在这些组之间有显著差异(p<；0.001，重复测量方差分析)。各照射组大鼠血清ROM值均在照射后16d出现峰值，与照射后可见皮肤反应的出现相对应。结论。血清ROM值可能有助于评估哺乳动物的辐射损伤。需要进一步的研究来从基因和蛋白质水平上研究辐射后细胞内代谢的变化。
Purpose. Ionizing radiation generates free radicals and reactive oxygen species that induce DNA damage in vivo. This study aimed to determine the relationship between serum reactive oxygen metabolite (ROM) levels and skin reaction after irradiation in a rat model. Methods and materials. I. Female Wistar rats were classified into 0 Gy (control), 2 Gy, and 30 Gy groups; serum ROM levels were measured in the very acute phase. II. Other female Wistar rats were classified into 0 Gy (control), 30 Gy, 50 Gy, and 70 Gy groups; serum ROM levels were measured before and 3, 7, 16, 24, 31, and 38 days after irradiation. Skin reaction was evaluated according to the SRS (0-5) twice every week. Results. Serum ROM levels in the subacute phase were significantly higher in the 50 and 70 Gy groups than in the 0 and 30 Gy groups [p = 0.029, repeated-measure analysis of variance (ANOVA)]. As expected, SRSs increased in the order of the 0 Gy, 30 Gy, 50 Gy, and 70 Gy groups and differed significantly among these groups (p < 0.001, repeated-measure ANOVA). Peak serum ROM levels were observed 16 days after irradiation in all irradiated groups and corresponded with the appearance of visible skin reaction after irradiation. Conclusions. Serum ROM levels may be useful for evaluating radiation damage in mammals. Further investigations are required to investigate changes in intracellular metabolism after irradiation at gene and protein levels.