GDF9 affects the development and tight junction functions of immature bovine Sertoli cells.
GDF9 affects the development and tight junction functions of immature bovine Sertoli cells.
复制标题
DOI：
10.1111/rda.12960
复制
发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Keqiong Tang;Lei Wang;Yaping Jin;Wucai Yang;Liguo Yang
中科院分区：
文献类型：
--
作者：
Keqiong Tang;Lei Wang;Yaping Jin;Wucai Yang;Liguo Yang
转化生长因子-β (tgf -β)超家族是生殖细胞发育的重要调节因子，作为信号转导途径的细胞外配体，调节增殖、凋亡和细胞行为的其他方面。生长分化因子9 （growth differentiation factor 9, GDF9）作为TGF-β超家族成员，在女性卵巢卵泡发育和排卵率中起关键作用；然而，它在睾丸中的作用尚未得到很好的阐明。本研究旨在探讨GDF9及其受体基因BMPRII和ALK5在青春期前牛支持细胞（SCs）中的表达。此外，我们还评估了GDF9对未成熟SCs凋亡、细胞周期和紧密连接功能的影响。我们发现GDF9及其受体基因BMPRII和ALK5在未成熟SCs中表达。外源性GDF9通过显著上调Cyclin E（细胞周期）和bcl-2（抗凋亡）mRNA表达，下调caspase-3（促凋亡）mRNA表达，显著促进SCs增殖，抑制SCs凋亡。同时，外源GDF9通过显著下调claudin-11 mRNA的表达，影响occludin的分布，显著降低了上皮电阻值。综上所述，本研究表明GDF9通过调节细胞周期、凋亡和紧密连接功能，是牛SCs的关键调节因子。
Transforming growth factor-β (TGFβ) superfamily are critical regulators of germ cell development that act as extracellular ligands of the signal transduction pathways regulating proliferation, apoptosis and other aspects of cell behaviour. As a member of the TGF-β superfamily, growth differentiation factor 9 (GDF9) plays a critical role in ovarian follicular development and the ovulation rate in females; however, its role in the testis has not been well elucidated. The aim of this study was to investigate the expression of GDF9 and its receptor genes BMPRII and ALK5 in prepuberal bovine Sertoli cells (SCs). In addition, we assessed the effects of GDF9 on immature SCs apoptosis, the cell cycle and tight junction functions. We found that GDF9 and its receptor genes BMPRII and ALK5 were expressed in immature SCs. Exogenous GDF9 significantly promoted SCs proliferation and inhibited the apoptosis of SCs by significantly upregulating Cyclin E (cell cycle) and bcl-2 (anti-apoptosis) mRNA expression and downregulating caspase-3 (pro-apoptosis) mRNA expression. Meanwhile, exogenous GDF9 significantly decreased the value of transepithelial electrical resistance by significantly downregulating claudin-11 mRNA expression and influencing the distribution of occludin. In conclusion, this study reveals that GDF9 is a key regulator of bovine SCs through the modulation of the cell cycle, apoptosis and tight junction functions.