Real-time quantitative RT-PCR after laser-assisted cell picking
Real-time quantitative RT-PCR after laser-assisted cell picking
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DOI：
10.1038/3327
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发表时间：
1998-11-01
期刊：
影响因子：
82.9
通讯作者：
Bohle, RM
中科院分区：
文献类型：
--
作者：
Fink, L;Seeger, W;Bohle, RM
新技术11.然而，影响因素在单个样品中变化很小，因此假设K相等，因此不影响计算的相对比值的比较。为了比较不同的运行，必须计算运行效率。PCR的效率提供了关于扩增速率的信息，并且在0到1之间变化。1（= 100%）意味着在每个循环中，拷贝量加倍。将PBGD的PCR产物以对应于挑选的细胞的拷贝数的浓度连续稀释，用于产生标准曲线。计算运行效率时需要该标准曲线的斜率。该关系由下式给出：
NEW TECHNOLOGY one11. However, affecting factors will vary only negligibly among single samples so that K is assumed to be equal and thus does not influence the comparison of calculated relative ratios. For comparison of different runs the run efficiencies have to be calculated. The efficiency of PCR provides information about the amplification rate and varies from 0 to 1. One (= 100%) means that in each cycle the amount of copies is doubled. A serial dilution of a PCR product of PBGD in concentrations corresponding to copy numbers of picked cells served for generating a standard curve. The slope of this standard curve is required for calculation of the run efficiency. The relation is given by: