Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection.
Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection.
复制标题
体内人bcl-2基因转染预防缺氧性肝细胞坏死。
DOI：
10.1006/bbrc.1997.7925
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发表时间：
1998
影响因子：
3.1
通讯作者：
Hikaru Matsuda
中科院分区：
文献类型：
--
作者：
Kazuo Yamabe;S. Shimizu;W. Kamiike;S. Waguri;Y. Eguchi;J. Hasegawa;Shinichirou Okuno;Y. Yoshioka;Toshinori Ito;Yoshiki Sawa;Y. Uchiyama;Y. Tsujimoto;Hikaru Matsuda
防止缺氧性细胞死亡是肝移植成功的关键。我们开发了一种通过将抗细胞死亡基因直接导入大鼠肝脏来预防肝脏缺氧性细胞死亡的新方法。用日本仙台病毒血凝病毒(HvJ)-脂质体方法将人bcl2基因(Hbcl2)与非组蛋白染色体高迁移率族蛋白1(HMG1)一起直接导入大鼠肝脏，可高效表达人bcl2蛋白。电子显微镜和荧光显微镜显示外源性hBcl2表达的肝细胞几乎完全保护了缺氧性细胞的坏死。HBcl2的表达也抑制了caspase-3(样蛋白)酶的激活和肝功能障碍。因此，通过HVJ-脂质体转导hbc1-2基因对预防缺氧性肝细胞坏死有一定的作用。这一发现可能导致新的策略，以避免缺氧性细胞死亡，这是肝移植的主要问题。
Prevention of hypoxic cell death is a key to successful liver transplantation. We developed a new method for preventing liver hypoxic cell death by introducing an anti-cell death gene directly into rat livers. When the human bcl-2 gene (hbcl-2) was directly transfected into rat livers together with non-histone chromosomal protein high mobility group 1 (HMG1) by the hemagglutinating virus of Japan (Sendai virus; HVJ)-liposome method, human Bcl-2 protein (hBcl-2) was efficiently expressed. Electron microscopy and fluorescence microscopy revealed that hepatocytes expressing exogenous hBcl-2 were almost completely protected the hypoxic cell necrosis. The expression of the hBcl-2 also inhibited activation of caspase-3 (-like) proteases and liver dysfunction. Thus, we conclude that transfection of the hbcl-2 gene through HVJ-liposome method is useful to prevent liver cell necrosis induced by hypoxia. This finding could lead to new strategies to avoid the hypoxic cell death, the major problem in liver transplantation.
环孢素和肉碱通过抑制线粒体通透性转变来防止培养的肝细胞缺氧死亡。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pastorino,JG;Snyder,JW;Serroni,A;Hoek,JB;Farber,JL
通讯作者：
Farber,JL