Immunolocalization of general odorant-binding protein in antennal sensilla of moth caterpillars
Immunolocalization of general odorant-binding protein in antennal sensilla of moth caterpillars
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DOI：
10.1016/s1467-8039(00)00013-x
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发表时间：
2000-01-01
影响因子：
2
通讯作者：
Laue, M
中科院分区：
文献类型：
--
作者：
Laue, M
用抗天蚕信息素结合蛋白和通用气味结合蛋白2的抗血清对家蚕和棉铃虫幼虫的触角进行免疫标记，以确定这些蛋白在个体感器中的表达，并与成虫感器中的定位模式进行比较。特异性标记的触角感器，只获得与一般气味结合蛋白2的抗血清。在天线上存在的几个感器中，三个大的basiconica感器，这被怀疑是嗅觉功能，被标记。这些感器是由多个感器单位组成的复合感器，它们形成一个共同的感觉毛。头发是单壁的，有许多毛孔。感器室的标记与成虫的感器相同。感器淋巴的突出标记伴随着两个最外的辅助细胞中的分泌细胞器和所有感器细胞中的内吞途径的标记。结果表明，一般气味结合蛋白表达在蛾幼虫触角上的单壁多孔感器中，推测其具有嗅觉功能。幼虫和成虫感受器之间的一般气味结合蛋白的定位模式的整体一致性暗示了这些蛋白在嗅觉刺激转导中的类似作用，（C）2000 Elsevier Science Ltd.保留所有权利。
Antennae of Bombyx mori and Helicoverpa armigera larvae were immunolabelled with antisera raised against the pheromone-binding protein or the general odorant-binding protein 2 of Antheraea polyphemus to assign the expression of these proteins to individual sensilla and to compare the localization pattern with that in sensilla of adult moths. Specific labelling of antennal sensilla was only obtained with the antiserum against general odorant-binding protein 2. Among the few sensilla present on the antenna the three Large sensilla basiconica, which are suspected to be olfactory in function, were labelled. These sensilla are compound sensilla consisting of several sensillum units which form a common sensory hair. The hair is single-walled and pierced by many pores. Labelling of sensillum compartments was the same as in sensilla of adults. Prominent labelling of the sensillum lymph is accompanied by labelling of secretory organelles in the two outermost auxiliary cells and of endocytotic pathways in all sensillum cells. The results suggest that general odorant-binding protein is expressed in single-walled multiporous sensilla of presumed olfactory function on the antenna of moth larvae. The overall identity of the localization pattern for general odorant-binding protein between larval and adult sensilla implies a similar role of these proteins in olfactory stimulus transduction, (C) 2000 Elsevier Science Ltd. All rights reserved.