Microbiology of striped bass grown in three aquaculture systems
Microbiology of striped bass grown in three aquaculture systems
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三种水产养殖系统中生长的条纹鲈的微生物学
DOI：
10.1006/fmic.1996.0095
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发表时间：
1997
影响因子：
5.3
通讯作者：
D. Westhoff
中科院分区：
文献类型：
--
作者：
P. C. Nedoluha;D. Westhoff
研究了3种养殖体系(土池、流槽和循环槽)对黑点石斑鱼(Morone Saxatilis)和杂交鱼(Morone chrysops×Morone saxatilus)皮肤(S)、鳃(G)、肠道内容物(I)和养殖水体(W)微生物数量和种类的影响。在35℃、22℃和7℃下进行需氧和厌氧平板计数；用MIDI和Biolog系统鉴定从需氧平板计数中随机选择的菌株(n=1742)。采用富集法和选择性平板法检测食源性致病菌。用混合模型方差分析确定平板计数之间的显著差异(P<；0.05)；分别对S、G、I和W的数据进行分析。养殖制度对S、G、I、W的菌落数无显著影响。在相同的培养温度下，S、G、W的好氧平板数显著高于厌氧菌数，而I、G、W组的好氧和厌氧菌数差异不显著。在相同的培养气氛下，35℃和22℃的细菌计数差异不显著，但这两个温度下的细菌计数均显著高于7℃的细菌计数。所有系统随机分离的细菌类型相似，但其流行率不同；培养温度影响所分离的属。从所有系统和组织中随机分离的主要微生物群为气单胞菌(19%)、Flavobacterium/Cytophaga/Chryseobacterium(16%)，芽孢杆菌(7%)、莫拉菌科(6%)、假单胞菌(6%)、角膜(5%)、邻单胞菌(5%)和微球菌科(4%)。未分离到沙门氏菌或小肠结肠炎耶尔森氏菌，单核细胞增生性李斯特氏菌、志贺氏菌痢疾和假结核耶尔森氏菌各只在一次采样日内从池塘中分离到。在所有系统中都以较低的数量发生。
The effects of three aquaculture systems (earthen ponds, flow-through tanks and recirculating tanks) on the numbers and types of micro-organisms isolated from the skin (S), gills (G), intestinal contents (I) and cultivation water (W) of striped bass (Morone saxatilis) and hybrid striped bass (Morone chrysops×Morone saxatilus) were determined. Plates counts were performed aerobically and anaerobically at 35, 22 and 7°C; randomly-selected isolates (n=1742) from the aerobic plate counts were identified using MIDI and BIOLOG systems. Enrichments and selective plating were used to detect foodborne pathogens. A mixed model analysis of variance was used to determine significant differences (P<0.05) among plate counts; data for the S, G, I and W were analysed separately. Aquaculture system had no significant effect on any of the plate counts for the S, G, I or W. Aerobic plate counts were significantly higher than anaerobic counts at the same incubation temperature for the S, G and W, but for the I, aerobic and anaerobic counts were not significantly different. Under the same incubation atmosphere, counts at 35 and 22°C were not significantly different from each other, but counts at both temperatures were significantly higher than counts at 7°C. The types of bacteria randomly isolated were similar for all systems, but their prevalence varied; incubation temperature affected the genera isolated. The predominant microbial groups randomly isolated from the fish samples from all systems and tissues wereAeromonas(19%),Flavobacterium/Cytophaga/Chryseobacterium(16%),Bacillus(7%), Moraxellaceae (6%),Pseudomonas(6%), corneforms (5%),Plesiomonas(5%) and Micrococcaceae (4%). NoSalmonellaorYersinia enterocoliticawere isolated, andListeria monocytogenes,Shigella dysenteriae, andYersinia pseudotuberculosiswere each isolated only from ponds on only one sampling day.Staphylococcus aureusandVibriospp. occurred in all systems in low numbers.