Lesional CD8+ T-Cell Number Predicts Surgical Outcomes of Melanocyte-Keratinocyte Transplantation Surgery for Vitiligo.
Lesional CD8+ T-Cell Number Predicts Surgical Outcomes of Melanocyte-Keratinocyte Transplantation Surgery for Vitiligo.
复制标题
病变 CD8 T 细胞数量可预测白癜风黑色素细胞-角质形成细胞移植手术的手术结果。
DOI：
10.1016/j.jid.2023.03.1689
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Harris,JohnE
中科院分区：
文献类型：
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作者：
Refat,MaggiA;Strassner,JamesP;Frisoli,MichaelL;Rashighi,Mehdi;Richmond,Jillian;Nada,Essam;Saleh,Ramadan;El-Hamd,MohammedAbu;Goldberg,Dori;Mahmoud,BasselH;Harris,JohnE
黑素细胞-角质形成细胞移植程序（MKTP）治疗稳定和复发性白癜风。尽管根据临床稳定性仔细选择候选人，但手术的成功是不可预测的。本研究的目的是确定接受MKTP治疗的稳定型白癜风皮损的免疫学特征，并将其与临床结局相关联。我们纳入了20名白癜风MKTP候选人和一名作为对照的斑驳病患者。在MKTP之前，通过流式细胞术和ELISA测量受体皮肤中的T细胞亚群和趋化因子。在MKTP期间，通过流式细胞术定量供体皮肤中的黑素细胞。在MKTP后，患者随访12个月，并通过临床照片的ImageJ分析对色素沉着进行临床评估。基线免疫学生物标志物、临床稳定性持续时间和移植黑素细胞数量与术后再色素沉着评分相关。CD 8 + T细胞在43%临床稳定的白癜风病变中升高。CD 8 + T细胞数量与术后色素再沉着评分呈负相关（r =-0.635，P = 0.002）。临床稳定性的持续时间、皮肤趋化因子和移植的黑素细胞数量不影响术后色素沉着。这项研究表明，CD 8 + T细胞数量与术后色素沉着的成功率呈负相关，可以作为识别理想手术候选人的生物标志物。
The melanocyte–keratinocyte transplantation procedure (MKTP) treats stable and recalcitrant vitiligo. Despite careful selection of candidates based on clinical stability, the success of the procedure is unpredictable. The aim of our study was to define the immunological profile of stable vitiligo lesions undergoing MKTP and correlate them with clinical outcomes. We included 20 MKTP candidates with vitiligo and a patient with piebaldism as a control. Prior to MKTP, T-cell subsets and chemokines in the recipient skin were measured by flow cytometry and ELISA. During MKTP, melanocytes in the donor skin were quantified by flow cytometry. After MKTP, patients were followed for 12 months and repigmentation was assessed clinically and by ImageJ analysis of clinical photographs. Baseline immunologic biomarkers, duration of clinical stability, and transplanted melanocyte number were correlated to postsurgical repigmentation scores. CD8+ T cells were elevated in 43% of the clinically stable vitiligo lesions. CD8+ T-cell number negatively correlated with postsurgical repigmentation scores (r = −0.635,P= 0.002). Duration of clinical stability, skin chemokines, and transplanted melanocyte number did not influence postsurgical repigmentation. This study demonstrates that CD8+ T-cell number correlates negatively with success of postsurgical repigmentation and can be a biomarker to identify ideal surgical candidates.