Hippocampal neurochemical and electrophysiological measures from freely moving rats.
Hippocampal neurochemical and electrophysiological measures from freely moving rats.
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自由活动大鼠的海马神经化学和电生理测量。
DOI：
10.1006/exnr.1998.6945
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Morgane,PJ
中科院分区：
文献类型：
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作者：
Bronzino,JD;Kehoe,P;Hendriks,R;Vita,L;Golas,B;Vivona,C;Morgane,PJ
本文描述了已开发的手术和记录程序，该程序允许同时监测选定的神经化学物质的水平（通过微透析）并测量自由活动的成年大鼠海马结构内的齿状诱发场电位。为了测试和评估这些程序，他们被用来检查与海马长时程增强（LTP）的建立和维持相关的海马神经化学和神经元兴奋性的变化。使用两种单独的强直范式在内侧穿支路径强直之前、期间和之后获得海马去甲肾上腺素（NE）和谷氨酸水平的测量以及齿状颗粒细胞群尖峰幅度（PSA）的测量。在几只动物中使用这些新程序获得的结果表明，NE 和谷氨酸水平的变化与破伤风后获得的齿状颗粒细胞 PSA 测量值的增加密切相关。结果表明，这种新开发的程序可以有效地用于直接检查与海马神经可塑性相关的神经化学和神经生理变化之间的关系。
This paper describes surgical and recording procedures that have been developed which permit the simultaneous monitoring of levels of select neurochemicals (via microdialysis) and measures of dentate-evoked field potentials within the hippocampal formation of freely moving adult rats. To test and evaluate these procedures, they were employed to examine changes in hippocampal neurochemistry and neuronal excitability associated with the establishment and maintenance of hippocampal long-term potentiation (LTP). Measures of hippocampal norepinephrine (NE) and glutamate levels along with measures of the dentate granule cell population spike amplitude (PSA) were obtained before, during, and after tetanization of the medial perforant path using two separate tetanization paradigms. Results obtained using these new procedures in several animals indicated that changes in NE and glutamate levels were strongly correlated with increases in the dentate granule cell PSA measure obtained following tetanization. The results indicate that this newly developed procedure can be effectively used to directly examine the relationship between neurochemical and neurophysiological changes associated with hippocampal neuroplasticity.