IDENTIFICATION OF THE PHAGE GENE FOR HOST RECEPTOR SPECIFICITY BY ANALYZING HYBRID PHAGES OF T5 AND BF23
IDENTIFICATION OF THE PHAGE GENE FOR HOST RECEPTOR SPECIFICITY BY ANALYZING HYBRID PHAGES OF T5 AND BF23
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DOI：
10.1016/0042-6822(84)90325-8
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
HELLER, KJ
中科院分区：
文献类型：
--
作者：
HELLER, KJ
亲缘关系密切的噬菌体T5和BF23在吸附到大肠杆菌细胞上所用的受体不同。为了鉴定编码宿主受体特异性的噬菌体基因，从T5和BF23的杂交中分离了杂交噬菌体。分析了这些杂交噬菌体的蛋白质组成、对不同细菌菌株的吸附动力学和DNA限制性内切酶图谱。对纯化尾巴的蛋白质组成分析表明，只有1条尾巴蛋白参与了宿主特异性的表达。在T5尾巴中，该蛋白被鉴定为一种明显分子量为67千道尔顿的次要尾蛋白。没有检测到相应的BF23蛋白。用限制性内切酶BamHI、HindIII和PstI对编码结构蛋白的DNA区域进行分析。这些重组子中限制酶切位点的分布表明，影响宿主特异性的区域位于apprx。T5基因组长度的90%。有证据表明，该基因与先前描述的OAD基因完全相同。对编码尾部蛋白的其他基因的位置进行了讨论。
The closely related coliphages T5 and BF23 differ in the receptor used for adsorption to Escherichia coli cells. To identify phage genes encoding host receptor specificity, hybrid phages were isolated from crosses of T5 and BF23. These hybrid phages were analyzed for their protein composition, kinetics of adsorption to various bacterial strains, and DNA restriction maps. Analyses of the protein compositions of purified tails suggested that only 1 tail protein was involved in the expression of host specificity. In T5 tails this protein was identified as a minor tail protein of an apparent MW of 67 kilodaltons. A corresponding BF23 protein could not be detected. The DNA region encoding structural proteins was analyzed by digestion with the restriction enzymes BamHI, HindIII and PstI. The distribution of restriction sites in these recombinants implied that the region affecting host specificity was located at .apprx. 90% of the T5 genome length. Evidence is presented that this gene is identical to the oad gene previously described. The location of other genes encoding tail proteins is discussed.