Molecular cloning of delta-4, a new mouse and human notch ligand
Molecular cloning of delta-4, a new mouse and human notch ligand
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DOI：
10.1093/oxfordjournals.jbchem.a002832
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Nishikawa, M
中科院分区：
文献类型：
--
作者：
Yoneya, T;Tahara, T;Nishikawa, M
分离出编码先前未鉴定的小鼠Notch配体及其人类直系同源物的互补DNA。新的Notch配体包含一个信号序列、一个DSL结构域、八个表皮生长因子样重复序列、一个跨膜结构域和一个细胞内区域，所有这些都是Delta蛋白家族成员的特征。因此，新蛋白被命名为 Delta-4。细胞外和细胞内区域中的几个先前未识别的序列被证明在脊椎动物 Delta 蛋白中是保守的。Delta-4 mRNA 的组织分布类似于先前描述的 Notch-4 (Int-3) 转录物。然而，与小鼠肺的原位杂交显示，邻近内皮细胞的鳞状肺泡细胞中富含 Delta-4 mRNA； Notch-4 表达主要限于后一种细胞类型。 Delta-4细胞外部分的可溶形式抑制人主动脉内皮细胞的明显增殖，但不抑制人肺动脉内皮细胞。
Complementary DNAs encoding a previously unidentified mouse Notch ligand and its human ortholog were isolated. The new Notch Ligand contains a signal sequence, a DSL domain, eight epidermal growth factor-like repeats, a transmembrane domain, and an intracellular region, all of which are characteristics of members of the Delta protein family. The new protein was therefore designated Delta-4, Several previously unidentified sequences in both the extracellular and intracellular regions were shown to be conserved among vertebrate Delta proteins, The tissue distribution of Delta-4 mRNA resembles that previously described for Notch-4 (Int-3) transcripts. However, in situ hybridization with mouse lung revealed that Delta-4 mRNA is abundant in squamous alveolar cells that neighbor endothelial cells; Notch-4 expression is largely restricted to the latter cell type. Soluble forms of the extracellular portion of Delta-4 inhibit the apparent proliferation of human aortic endothelial cells, but not human pulmonary arterial endothelial cells.