Altered expression of the retinoblastoma (RB) gene in small-cell carcinoma of the lung.
Altered expression of the retinoblastoma (RB) gene in small-cell carcinoma of the lung.
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发表时间：
1988
期刊：
影响因子：
8
通讯作者：
J. Yokota;T. Akiyama;Y. Fung;W. Benedict;Y. Namba;M. Hanaoka;M. Wada;T. Terasaki;Y. Shimosata;Takashi Sugimura;M. Terada
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作者：
J. Yokota;T. Akiyama;Y. Fung;W. Benedict;Y. Namba;M. Hanaoka;M. Wada;T. Terasaki;Y. Shimosata;Takashi Sugimura;M. Terada
用互补DNA探针检测了9个肺小细胞癌（SCC）细胞系和9个肺非SCC细胞系视网膜母细胞瘤（RB）基因的结构变化及其表达。使用我们生产的抗RB抗体研究RB蛋白产物。用RB cDNA探针和13号染色体多态性DNA标记进行Southern印迹分析，发现9例SCC中8例RB基因为纯合性或半合性，2例大细胞癌（LCC）中1例RB基因为半合性，但在这18个细胞系中未发现明显的RB基因结构改变。然而，RB转录本的数量显着减少或异常的长度在9 SCC中的3。特异性115 kD蛋白在所有9个RB mRNA大小正常或异常的SCC中均未被抗RB抗体免疫沉淀。4个腺癌（AdCs）中的3个，所有3个鳞状细胞癌，和2个LCCs中的1个表达正常大小的RB mRNA，和115 kD的蛋白被抗RB抗体免疫沉淀。在RB mRNA表达缩短的2例LCC和RB mRNA表达水平低的4例AdCs中，有1例缺失115 kD蛋白。这些结果有力地表明RB基因的失活可能参与了肺癌，特别是SCC的发展。
Nine lung small-cell carcinoma (SCC) cell lines and 9 lung non-SCC cell lines were examined for structural changes of the retinoblastoma (RB) gene as well as its expression using a complementary DNA probe. The RB protein product was investigated using an anti-RB antibody which we produced. Although homozygosity or hemizygosity of the RB gene was suggested in 8 of 9 SCCs and one of 2 large cell carcinomas (LCCs) by Southern blot analysis using an RB cDNA probe and polymorphic DNA markers for chromosome 13, no obvious structural changes of the RB gene were detected in these 18 cell lines. However, RB transcripts were either markedly reduced in quantity or abnormal in length in 3 of 9 SCCs. The specific 115 kD protein was not immunoprecipitated by the anti-RB antibody in all 9 SCCs with either normal or abnormal size RB mRNA. Three of 4 adenocarcinomas (AdCs), all 3 squamous cell carcinomas, and one of 2 LCCs expressed normal size RB mRNA, and the 115 kD protein was immunoprecipitated by the anti-RB antibody. The 115 kD protein was also absent in one of 2 LCCs with shortened RB mRNA and in one of 4 AdCs with low level of RB mRNA expression. These results strongly suggest that inactivation of the RB gene might be involved in the development of lung cancers, especially of SCCs.