Systemic administration of adrenomedullin(27-52) increases bone volume and strength in male mice
Systemic administration of adrenomedullin(27-52) increases bone volume and strength in male mice
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DOI：
10.1677/joe.0.1700251
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发表时间：
2001-07-01
影响因子：
4
通讯作者：
Reid, IR
中科院分区：
文献类型：
--
作者：
Cornish, J;Callon, KE;Reid, IR
肾上腺髓质素是一种由52个氨基酸组成的肽，最初在人类嗜铬细胞瘤中发现，但后来发现它存在于许多组织中，包括血管系统和骨骼。由于其结构相似的胰淀素和降钙素基因相关肽，这两者都对骨细胞的行动，我们以前评估了肾上腺髓质素对骨骼的影响，并发现它增加成骨细胞在体外增殖和骨形成后，在体内局部注射。本研究通过评估缺乏血管舒张活性结构要求的这种肽的片段的全身给药对骨的影响来推进这项工作。两组20只成年雄性小鼠在4周内接受20次注射人肾上腺髓质素（27-52）8.1 μ g或载体，并评估骨组织形态计量学和强度。在胫骨中，肾上腺髓质素（27-52）使成骨细胞活性指数、类骨质周长和成骨细胞周长增加（P
Adrenomedullin is a 52-amino acid peptide first described in a human phaeochromocytoma but since been found to be present in many tissues, including the vascular system and bone. Because of its structural similarity to amylin and calcitonin gene-related peptide, both of which have actions on bone cells, we have previously assessed the effects of adrenomedullin on the skeleton, and found that it increases osteoblast proliferation in vitro and bone formation following local injection in vivo. The present study carries this work forward by assessing the effects on bone of the systemic administration of a fragment of this peptide lacking the structural requirements for vasodilator activity. Two groups of 20 adult male mice received 20 injections of human adrenomedullin(27-52) 8.1 mug or vehicle over a 4-week period and bone histomorphometry and strength were assessed.In the tibia, adrenomedullin(27-52) produced increases in the indices of osteoblast activity, osteoid perimeter and osteoblast perimeter (P