Expression of EphA2 and VEGF in squamous cell carcinoma of the tongue: Correlation with the angiogenesis and clinical outcome
Expression of EphA2 and VEGF in squamous cell carcinoma of the tongue: Correlation with the angiogenesis and clinical outcome
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EphA2和VEGF在舌鳞状细胞癌中的表达：与血管生成和临床结果的相关性
DOI：
10.1016/j.oraloncology.2008.01.018
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发表时间：
2008-12-01
期刊：
影响因子：
4.8
通讯作者：
Shang, Zheng-Jun
中科院分区：
文献类型：
--
作者：
Shao, Zhe;Zhang, Wen-Feng;Shang, Zheng-Jun
Eph-ephrin结合已经与肿瘤生物学相关联，并且已经报道VEGF参与由Eph-ephrin调节的肿瘤血管生成。本研究旨在评价EphA 2和VEGF在口腔舌鳞癌中的表达与血管生成和临床预后的关系。免疫组织化学。采用免疫组化染色法检测59例手术切除的舌癌和10例无瘤粘膜中EphA 2和VEGF的蛋白表达水平。在所有病例中，通过计数CD 34反应性内皮细胞来评估微血管密度（MVD）。细胞或内皮细胞。细胞簇舌鳞癌组织中EphA 2和VEGF的表达均显著高于正常舌粘膜组织（P < 0.01）。MVD与EphA 2、VEGF表达呈显著相关（P < 0.01）。EphA 2、VEGF和MVD与肿瘤大小、临床分期、淋巴结转移、复发和远处转移相关（P < 0.05）。多因素分析显示EphA 2、VEGF表达、MVD和临床分期对预后有独立影响。生存结论：EphA 2和VEGF的过度表达与舌鳞癌的恶性程度有关。临床结果表明，这些蛋白的过表达可能有助于肿瘤血管生成，并在舌癌中具有预后价值。(C)2008 Elsevier Ltd.保留所有权利。
Eph-ephrin binding has been linked to tumor biology and VEGF has been reported to participate in the tumor angiogenesis regulated by Eph-ephrin. The present study was designed to evaluate the expression of EphA2 and VEGF in relation to angiogenesis and clinical outcome in squamous cell carcinoma of oral tongue. Immunohistochemical. staining was used to determine the protein expression levels of EphA2 and VEGF in 59 surgically resected tongue carcinomas and 10 tumor-free mucosas. In all, cases, microvessel density (MVD) was evaluated by counting CD34-reactive endothelial. cells or endothelial. cell clusters. Both EphA2 and VEGF staining activities in squamous cell carcinoma of oral tongue were more significant than those in normal mucosa (P < 0.01). MVD had significant correlations with EphA2 and VEGF expression (P < 0.01). The EphA2, VEGF, and MVD were significantly correlated with tumor size, clinical stage, lymph invasion, recurrence, and distant metastasis (P < 0.05). Multivariate analysis showed EphA2, VEGF expression, MVD, and clinical stage had an independent prognostic effect on overall. survival. We conclude that the overexpression of EphA2 and VEGF are related to malignancy in squamous cell carcinoma of oral tongue. Clinical outcomes raised the possibility that the overexpression of those proteins might contribute to tumor angiogenesis and have prognostic value in tongue cancer. (C) 2008 Elsevier Ltd. ALL rights reserved.