Electrical impedance myography for the in vivo and ex vivo assessment of muscular dystrophy (mdx) mouse muscle.
Electrical impedance myography for the in vivo and ex vivo assessment of muscular dystrophy (mdx) mouse muscle.
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DOI：
10.1002/mus.24086
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发表时间：
2014-06
期刊：
影响因子：
3.4
通讯作者：
Rutkove SB
中科院分区：
文献类型：
--
作者：
Li J;Geisbush TR;Rosen GD;Lachey J;Mulivor A;Rutkove SB
需要灵敏的、非侵入性的技术来提供疾病状态的生物标志物和肌营养不良症的治疗效果。我们通过研究2个月大和18个月大的mdx和野生型（WT）动物（4组各10只动物），评估了电阻抗肌电图（EIM）在这方面的作用。观察到mdx和WT动物之间的EIM值存在显著差异;年龄较大组之间的差异更明显（例如，mdx动物的电抗为92.6 ±4.3 Ω，WT动物的电抗为130 ± 4.1 Ω，P < 0.001）。此外，体内EIM参数与mdx动物中结缔组织沉积的程度显著相关。EIM有可能作为一个有价值的非侵入性方法评估肌营养不良症。它可以是一种有用的生物标志物，以协助临床前和临床研究中的治疗测试。
Sensitive, non-invasive techniques are needed that can provide biomarkers of disease status and the effects of therapy in muscular dystrophy. We evaluated electrical impedance myography (EIM) to serve in this role by studying 2-month-old and 18-month-old mdx and wild-type (WT) animals (10 animals in each of 4 groups). Marked differences were observed in EIM values between mdx and WT animals; the differences were more pronounced between the older age groups (e.g., reactance of 92.6 ±4.3 Ω for mdx animals vs. 130 ± 4.1 Ω for WT animals, P < 0.001). In addition, in vivo EIM parameters correlated significantly with the extent of connective tissue deposition in the mdx animals. EIM has the potential to serve as a valuable non-invasive method for evaluating muscular dystrophy. It can be a useful biomarker to assist with therapeutic testing in both preclinical and clinical studies.