Simultaneous determination of mecamylamine, nicotine, and cotinine in plasma by gas chromatography-mass spectrometry
Simultaneous determination of mecamylamine, nicotine, and cotinine in plasma by gas chromatography-mass spectrometry
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DOI：
10.1016/s0731-7085(00)00343-5
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发表时间：
2000-09-01
影响因子：
3.4
通讯作者：
Benowitz, NL
中科院分区：
文献类型：
--
作者：
Jacob, P;Wu, S;Benowitz, NL
尼古丁受体拮抗剂甲乙胺已被证明可以提高尼古丁作为烟草成瘾药物治疗的疗效。一种同时透皮给药的产品尼古丁和甲基苯丙胺正在进行临床试验。为了进行药代动力学研究，需要对甲氨基甲胺和尼古丁的每毫升低纳克水平进行定量。建立了用气相色谱-质谱仪(GC-MS)同时测定人血浆中甲氨基甲胺、尼古丁和尼古丁代谢物可替宁的方法。甲氨基甲胺、尼古丁和可替宁的定量限分别为2、1和2 ng/ml。(C)2000 Elsevier Science B.V.保留所有权利。
The nicotine receptor antagonist mecamylamine has been shown to increase the efficacy of transdermal nicotine as a pharmacotherapy for tobacco addiction. A product for simultaneous transdermal administration of nicotine and mecamylamine is undergoing clinical trials. In order to carry out pharmacokinetic studies, quantitation of low nanogram per milliliter levels of mecamylamine and nicotine was required. This paper describes a method for simultaneous determination of mecamylamine, nicotine, and the nicotine metabolite, cotinine, in human plasma using gas chromatography-mass spectrometry (GC-MS). Limits of quantitation for mecamylamine, nicotine and cotinine are 2, 1 and 2 ng/ml, respectively. (C) 2000 Elsevier Science B.V. AII rights reserved.