Microbial structure and chemical components of aerosols caused by rotating brushes in a wastewater treatment plant
Microbial structure and chemical components of aerosols caused by rotating brushes in a wastewater treatment plant
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污水处理厂旋转刷引起的气溶胶的微生物结构和化学成分
DOI：
10.1007/s11356-012-0885-1
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发表时间：
2012-11-01
影响因子：
5.8
通讯作者：
Zhang, Mengzhu
中科院分区：
文献类型：
--
作者：
Han, Yunping;Li, Lin;Zhang, Mengzhu
目的对北京市某污水处理厂Orbal氧化沟中旋转刷产生气溶胶的细菌群落结构和化学成分进行了研究。使用分子培养独立的方法来表征每个样品中的空气中的细菌的群落结构，而不管细胞的可培养性。利用16 S rDNA直接扩增技术构建了16 S rDNA克隆文库，并进行了序列测定，以分析其群落组成和多样性。通过扫描电子显微镜结合能量色散X射线光谱和离子色谱分析仪分析了气溶胶中微生物发出的不溶性颗粒和水溶性离子。旋转刷附近的序列（生物气溶胶的主要来源）以变形菌门（62.97%）和拟杆菌门（29.63%）为主，其中β-亚门（18.52%）和γ-亚门（44.45%）占绝大多数。复杂的模式，观察到每个采样位置，表明一个高度多样化的社区结构，在奥巴尔氧化沟的水相媲美。伴随微生物，SO 42 − 46.36 μg/m3，Cl− 29.35 μg/m3，NO3− 21.51 μg/m3，NH 4 + 19.76 μg/m3，PO 43 − 11.42 μg/m3，NO2− 6.18 μg/m3，Mg、Cl、K、Na、Fe、S等元素，结论不同采样点气溶胶中细菌群落结构和化学成分存在差异，说明不同采样点气溶胶中细菌群落结构和化学成分存在差异重要的站点相关的可变性。水体中的微生物组成是生物气溶胶中细菌群落的重要来源之一。生物气溶胶中的化学成分可以为空气微生物的附着提供媒介，以及为它们在空气中的生长和生存提供合适的微环境。本研究将有助于制定污染标准，特别是气溶胶，考虑到工厂工人的健康。
PurposeBacterial community structure and the chemical components in aerosols caused by rotating brushes in an Orbal oxidation ditch were assessed in a Beijing municipal wastewater treatment plant.MethodsAir samples were collected at different distances from the aerosol-generating rotating brushes. Molecular culture-independent methods were used to characterize the community structure of the airborne bacteria in each sample regardless of cell culturability. A clone library of 16S rDNA directly amplified from air DNA of each sample was constructed and sequenced to analyze the community composition and diversity. Insoluble particles and water-soluble ions emitted with microorganisms in aerosols were analysis by a scanning electron microscope together with energy dispersive X-ray spectroscopy and ion chromatogram analyzer.ResultsIn total, most of the identified bacteria were Proteobacteria. The majority of sequences near the rotating brushes (the main source of the bioaerosols) were Proteobacteria (62.97 %) with β-(18.52 %) and γ-(44.45 %) subgroups and Bacteroidetes (29.63 %). Complex patterns were observed for each sampling location, suggesting a highly diverse community structure, comparable to that found in water in the Orbal oxidation ditch. Accompany with microorganisms, 46.36 μg/m3of SO42−, 29.35 μg/m3of Cl−, 21.51 μg/m3of NO3−, 19.76 μg/m3of NH4+, 11.42 μg/m3of PO43−, 6.18 μg/m3of NO2−, and elements of Mg, Cl, K, Na, Fe, S, and P were detected from the air near the aerosols source.ConclusionsDifferences in the structure of the bacterial communities and chemical components in the aerosols observed between sampling sites indicated important site-related variability. The composition of microorganisms in water was one of the most important sources of bacterial communities in bioaerosols. Chemical components in bioaerosols may provide a media for airborne microorganism attachment, as well as a suitable microenvironment for their growth and survival in the air. This study will be benefit for the formulation of pollution standards, especially for aerosols, that take into account plant workers’ health.