Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations
Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations
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DOI：
10.1002/bit.23283
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发表时间：
2012-01-01
影响因子：
3.8
通讯作者：
Sinskey, Anthony J.
中科院分区：
文献类型：
--
作者：
Riedel, Sebastian L.;Bader, Johannes;Sinskey, Anthony J.
生产成本的提高将加速聚羟基链烷酸酯（PHA）聚合物和PHA基产品的商业化。植物油被认为是有利的原料，因为与糖和其他精炼碳原料相比，它们的碳含量高且价格相对较低。使用真养罗尔斯通氏菌的重组菌株比较了不同的PHA生产策略，该重组菌株在植物油上生长时产生大量的P（HB-共-HHx）。这个R。使用分批、延长分批和补料分批发酵策略使真养型菌株生长至高细胞密度，其中PHA积累由氮限制引发。虽然延长的分批培养比分批培养产生更多的生物质和PHA，但补料分批培养显示产生最高水平的生物质和PHA。达到的最高滴度超过139 g/L细胞干重（CDW）的生物质，其中74%的CDW为含有19mol%HHx的PHA。我们的数据表明，发酵过程是可扩展的，时空产率（STY）优于1 g PHA/L/h。所获得的生物量浓度和PHA产率是重组R.产P（HB-co-HHx）的真养型菌株。Biotechnol. Bioeng. 2012;109：7483。(c)2011 Wiley Periodicals，Inc.
Improved production costs will accelerate commercialization of polyhydroxyalkanoate (PHA) polymer and PHA-based products. Plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. Different PHA production strategies were compared using a recombinant strain of Ralstonia eutropha that produces high amounts of P(HB-co-HHx) when grown on plant oils. This R. eutropha strain was grown to high cell densities using batch, extended batch, and fed batch fermentation strategies, in which PHA accumulation was triggered by nitrogen limitation. While extended batch culture produced more biomass and PHA than batch culture, fed batch cultivation was shown to produce the highest levels of biomass and PHA. The highest titer achieved was over 139 g/L cell dry weight (CDW) of biomass with 74% of CDW as PHA containing 19mol% HHx. Our data suggest that the fermentation process is scalable with a space time yield (STY) better than 1 g PHA/L/h. The achieved biomass concentration and PHA yield are among the highest reported for the fermentation of recombinant R. eutropha strains producing P(HB-co-HHx). Biotechnol. Bioeng. 2012;109: 7483. (c) 2011 Wiley Periodicals, Inc.