Principles of bone marrow transplantation (BMT): providing optimal veterinary and husbandry care to irradiated mice in BMT studies.
Principles of bone marrow transplantation (BMT): providing optimal veterinary and husbandry care to irradiated mice in BMT studies.
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发表时间：
2009
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通讯作者：
R. Duran‐Struuck;R. Dysko
中科院分区：
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作者：
R. Duran‐Struuck;R. Dysko
骨髓移植(BMT)是许多白血病、实体瘤和代谢性疾病的首选疗法。骨髓研究领域高度依赖体内实验，因为体外技术不能模拟这些复杂的体内系统。因此，了解这些短暂免疫缺陷的骨髓受体动物的医疗和畜牧业护理需求对研究人员、兽医和动物护理人员至关重要。在这里，我们讨论骨髓移植的原理，可能干扰移植研究的小鼠病原体，以及可能有助于将移植过程中不必要的感染降至最低的重要饲养和兽医实践。全身照射是受者骨髓清髓最常用的工具之一。我们讨论了辐照器对骨髓移植研究的关键作用，以及无菌饲养实践的重要性，以减少辐照器成为疾病传播源的可能性。最后，我们讨论了机构动物使用和护理委员会审查辐射和BMT方案的一些重要指导方针。
Bone marrow transplantation (BMT) is the treatment of choice for many leukemias, solid tumors, and metabolic diseases. The field of bone marrow research is highly dependent on in vivo experimentation, because in vitro techniques do not mimic these complicated in vivo systems. Therefore, understanding the medical and husbandry care needs of these transiently immunodeficient bone marrow recipient animals is crucial for researchers, veterinary and animal care personnel. Here we discuss the principles of bone marrow transplantation, mouse pathogens that can interfere with transplantation research, and important husbandry and veterinary practices for mice that may help to minimize unnecessary infections during the transplantation process. Whole-body irradiation is one of the most common tools for myeloablation of the recipient's bone marrow. We discuss the crucial role of the irradiator for BMT research and the importance of aseptic husbandry practices to lessen the possibility of the irradiator for being a source for disease transmission. Finally, we discuss some important guidelines for Institutional Animal Use and Care Committees reviewing irradiation and BMT protocols.