Upconversion fluorescence-SERS dual-mode tags for cellular and in vivo imaging.
Upconversion fluorescence-SERS dual-mode tags for cellular and in vivo imaging.
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DOI：
10.1021/am500411m
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发表时间：
2014-03
影响因子：
9.5
通讯作者：
Xiaojuan Niu;Haiyan Chen;Yunqing Wang;Wenhai Wang;Xiuyan Sun;Lingxin Chen
中科院分区：
文献类型：
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作者：
Xiaojuan Niu;Haiyan Chen;Yunqing Wang;Wenhai Wang;Xiuyan Sun;Lingxin Chen
荧光表面增强拉曼散射（F-SERS）双模标签具有直观、快速的荧光成像和SERS技术的多路复用能力的综合优势，在生物成像方面具有很大的潜力。在以往报道的F-SERS标签中，一般选择有机荧光染料或量子点来产生荧光信号。在此，我们报道了基于近红外（NIR）激光（980 nm）激发的上转换纳米粒子（UCNPs）的上转换荧光(UCF)-SERS双模式标签的概念验证，用于活细胞和体内成像。该标签涉及三个成分：NaYF4:Yb，Er UCNPs@SiO2作为标签的荧光核心；在UCNPs@SiO2表面原位生长银纳米粒子，产生特征拉曼信号；变性BSA涂层使标签具有稳定性和生物相容性。UCF-SERS标签结合了荧光UCNPs和等离子体SERS纳米探针的近红外成像能力，促进了双模式生物成像研究，特别是对活体动物。离体实验表明，在980 nm和785 nm的近红外激光照射下，标签的UCF和SERS信号分别可以在3 mm和7 mm深的猪肉组织中检测到。此外，UCF-SERS标签在活体小鼠上的体内成像能力也得到了成功验证。所开发的双模态标签在医学诊断和治疗方面具有巨大的潜力。
Fluorescent-surface enhanced Raman scattering (F-SERS) dual mode tags showed great potential for bioimaging due to the combined advantages of intuitive, fast imaging of fluorescence and multiplex capability of SERS technique. In previously reported F-SERS tags, organic fluorescent dyes or quantum dots were generally selected to generate fluorescence signal. Herein, we reported the first proof-of-concept upconversion fluorescence (UCF)-SERS dual mode tags based on near infrared (NIR) laser (980 nm) excited upconversion nanoparticles (UCNPs) for live-cell and in vivo imaging. Three components involved in this tag: NaYF4:Yb,Er UCNPs@SiO2 serving as the fluorescent core of the tag; silver nanoparticles in situ grown on the surface of UCNPs@SiO2 for generating characteristic Raman signal; and denatured BSA coating rendering the tag's stability and biocompatibility. The UCF-SERS tags integrated the NIR imaging capability of both fluorescent UCNPs and plasmonic SERS nanoprobe, which facilitated dual mode bioimaging investigation, especially for living animals. Ex vivo experiments revealed that with 980 nm and 785 nm NIR laser irradiations, the UCF and SERS signals of the tags could be detected from 3 and 7 mm deep pork tissues, respectively. Furthermore, the in vivo imaging capabilities of UCF-SERS tags were successfully demonstrated on living mice. The developed dual modality tags held great potential for medical diagnostics and therapy.