ALTERED SARCOPLASMIC-RETICULUM CA-2+-ATPASE GENE-EXPRESSION IN THE HUMAN VENTRICLE DURING END-STAGE HEART-FAILURE
ALTERED SARCOPLASMIC-RETICULUM CA-2+-ATPASE GENE-EXPRESSION IN THE HUMAN VENTRICLE DURING END-STAGE HEART-FAILURE
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DOI：
10.1172/jci114429
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发表时间：
1990-01-01
影响因子：
15.9
通讯作者：
SCHWARTZ, K
中科院分区：
文献类型：
--
作者：
MERCADIER, JJ;LOMPRE, AM;SCHWARTZ, K
最近有报道在实验性心肌肥厚和衰竭时，心肌肌浆网（SR）中编码Ca ~（2+）-ATP酶的mRNA水平降低。为了确定这种缺陷是否发生在人类终末期心力衰竭中，我们使用大鼠心脏SR Ca 2 +-ATP酶cDNA探针比较了13例接受心脏移植的患者（6例特发性扩张型心肌病; 4例冠状动脉疾病伴心肌梗死; 3种不同病因）的左心室（LV）和右心室（RV）标本中SR Ca 2 +-ATP酶mRNA水平与对照心脏样本。我们观察到与对照组相比，心力衰竭患者左室标本中Ca 2 +-ATP酶mRNA相对于18 S核糖体RNA和肌球蛋白重链mRNA显著降低（分别为-48%，P < 0.01和-47%，P < 0.05）。左心室Ca ~（2+）-ATP酶mRNA/18 S RNA比值与心指数呈正相关（P < 0.02）。RV比值与肺动脉收缩压、舒张压和平均肺动脉压呈负相关（分别为P < 0.02、P < 0.02和P < 0.01）。我们认为，减少SR Ca ~（2+）-ATP酶mRNA在心肌中起着重要的作用，在人类终末期心力衰竭期间的Ca ~（2+）运动和心肌舒张的改变报告。
A decrease in the myocardial level of the mRNA encoding the Ca2+-ATPase of the sarcoplasmic reticulum (SR) has been recently reported during experimental cardiac hypertrophy and failure. To determine if such a deficit occurs in human end-stage heart failure, we compared the SR Ca2+-ATPase mRNA levels in left (LV) and right ventricular (RV) specimens from 13 patients undergoing cardiac transplantation (6 idiopathic dilated cardiomyopathies; 4 coronary artery diseases with myocardial infarctions; 3 diverse etiologies) with control heart samples using a rat cardiac SR Ca2+-ATPase cDNA probe. We observed a marked decrease in the mRNA for the Ca2+-ATPase relative to both the 18S ribosomal RNA and the myosin heavy chain mRNA in LV specimens of patients with heart failure compared to controls (-48%, P < 0.01 and -47%, P < 0.05, respectively). The LV ratio of Ca2+-ATPase mRNA to 18S RNA positively correlated with cardiac index (P < 0.02). The RV ratio correlated negatively with systolic, diastolic and mean pulmonary arterial pressures (P < 0.02, P < 0.02, and P < 0.01, respectively). We suggest that a decrease of the SR Ca2+-ATPase mRNA in the myocardium plays an important role in alterations of Ca2+ movements and myocardial relaxation reported during human end-stage heart failure.