The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)
The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)
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DOI：
10.1046/j.1365-294x.1999.00627.x
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发表时间：
1999-05-01
影响因子：
4.9
通讯作者：
Bussell, JD
中科院分区：
文献类型：
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作者：
Bussell, JD
从六个种群的Isotoma petraea F Muell的植物中产生RAPD。该物种生活在澳大利亚南部和西部的岩石露头上，种群表现出不同的繁殖系统，包括完全的自婚，不同程度的远系繁殖和复杂的杂交。随机扩增多态性DNA（RAPD）数据集的非度量多维标度（nMDS）分析清楚地解决了所有人群。Pigeon Pock种群是复杂杂种和结构纯合子植物的家园，通过nMDS分析将其分为这两组。有一点多样性，在高度自体交配的人群，但水平较高的远交Yackeyackine人口。所有复杂的杂种群体和植物都具有许多遗传系统特异性的RAPD，其中一些表现为固定的杂合性。估计GS，使用RAPDs一直有问题，由于其优势，和分析方法通常依赖于知识的自交率或假设哈迪-温伯格平衡。当种群表现出固定的杂合性时，这种假设不成立，而另一种方法，香农指数，被用来划分遗传多样性。遗传多样性的分布符合预期的近亲繁殖的物种，大部分的变化（87.5%）发生在种群之间。相比之下，平均基于RAPD的G（ST）为59.6%的近亲繁殖物种和15.5%的远交物种。
RAPDs were generated from plants of six populations of Isotoma petraea F Muell. The species occurs on rock outcrops in southern and western Australia, with populations exhibiting different breeding systems, including complete autogamy, varying levels of outbreeding and complex hybridity. Non-metric multidimensional scaling (nMDS) analysis of the random amplified polymorphic DNA (RAPD) data set clearly resolved all populations. The Pigeon Pock population, which is home to both complex hybrid and structural homozygote plants, was divided into those two groups by the nMDS analysis. There was little diversity in highly autogamous populations, but levels were higher in the outbred Yackeyackine population. All complex hybrid populations and plants possessed numerous genetic system-specific RAPDs, some of which were shown to be held in fixed heterozygosity. Estimating GS, using RAPDs has been problematical due to their dominance, and analytical methods usually rely on knowledge of the selfing rate or assume Hardy-Weinberg equilibrium. This assumption does not hold when populations exhibit fixed heterozygosity, and an alternative method, Shannon's Index, was used to partition genetic diversity. The distribution of genetic diversity fit expectations for an inbreeding species, with most of the variation (87.5%) occurring between populations. This compares to an average RAPD-based G(ST) of 59.6% for inbreeding species generally and 15.5% for outbreeding species.