Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest
Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest
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DOI：
10.1002/bit.26270
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发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
Mermod, Nicolas
中科院分区：
文献类型：
--
作者：
Droz, Xuan;Harraghy, Niamh;Mermod, Nicolas
我们开发了一种用于快速分选和选择高水平表达和分泌蛋白质的哺乳动物细胞的方法。该程序依赖于使用处理抗体偶联的磁性微粒的自动化微流体装置的细胞捕获，以及捕获后细胞从微粒的定时释放。使用临床上兼容的材料和程序，我们表明这种方法能够区分真正分泌大量蛋白质的细胞与那些只是在其表面上以高水平显示蛋白质而没有适当释放蛋白质的细胞。这种方法允许鉴定分泌高水平治疗蛋白的CHO细胞克隆，而如果没有细胞分选程序，这是无法实现的。Biotechnol. Bioeng. 2017;114：1791-1802. (c)2017年Wiley Periodicals，Inc.
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure. Biotechnol. Bioeng. 2017;114: 1791-1802. (c) 2017 Wiley Periodicals, Inc.