ProbeMaker: an extensible framework for design of sets of oligonucleotide probes.

ProbeMaker: an extensible framework for design of sets of oligonucleotide probes.
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探针制造商:用于设计一组寡核苷酸探针的可扩展框架。

DOI:
10.1186/1471-2105-6-229
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发表时间:
2005-09-19
期刊:
影响因子:
3
通讯作者:
Landegren, U
Landegren, U
中科院分区:
生物学4区
文献类型:
--
作者:
Stenberg, J;Nilsson, M;Landegren, U

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基于寡核苷酸探针的遗传分析程序是一种强大的工具,可以对遗传物质进行高度平行的研究。这样的程序需要使用特定于应用的设计约束来设计大型探头集。ProbeMaker是一种用于计算机辅助设计和分析寡核苷酸探针序列集的软件框架。该工具有助于设计用于靶序列集合的探针,包括用于扩增、可视化或鉴定目的的序列基序。扩展系统允许框架配备用于评估探针序列的应用特定组件,并提供包括对从各种文件格式导入序列数据的支持的可能性。ProbeMaker适合于许多不同的寡核苷酸设计和分析任务,包括用于各种类型的并行遗传分析的探针组的设计、设计参数的实验验证以及探针序列评估算法的电子测试。
Procedures for genetic analyses based on oligonucleotide probes are powerful tools that can allow highly parallel investigations of genetic material. Such procedures require the design of large sets of probes using application-specific design constraints. ProbeMaker is a software framework for computer-assisted design and analysis of sets of oligonucleotide probe sequences. The tool assists in the design of probes for sets of target sequences, incorporating sequence motifs for purposes such as amplification, visualization, or identification. An extension system allows the framework to be equipped with application-specific components for evaluation of probe sequences, and provides the possibility to include support for importing sequence data from a variety of file formats. ProbeMaker is a suitable tool for many different oligonucleotide design and analysis tasks, including the design of probe sets for various types of parallel genetic analyses, experimental validation of design parameters, and in silico testing of probe sequence evaluation algorithms.
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