Peptide-Mediated Electrochemical Steric Hindrance Assay for One-Step Detection of HIV Antibodies
Peptide-Mediated Electrochemical Steric Hindrance Assay for One-Step Detection of HIV Antibodies
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DOI：
10.1021/acs.analchem.9b00648
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发表时间：
2019-04-16
影响因子：
7.4
通讯作者：
Kelley, Shana O.
中科院分区：
文献类型：
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作者：
Mahshid, Sahar Sadat;Mahshid, Sara;Kelley, Shana O.
通过检测免疫系统产生的特异性抗体，可以诊断患者的传染病，包括人类免疫缺陷病毒（HIV）感染。我们以前已经表明，大分子，如抗体，可以有效地检测复杂的生物样品通过空间抑制杂交的共轭互补DNA链的电极结合的DNA链。在这里，我们报告了肽介导的电化学空间位阻杂交法，PeSHHA，专门用于检测针对HIV-1的gp41蛋白的抗体。我们表明，这种PeSHHA的灵敏度可以显着提高使用纳米结构的电极，并证明了快速，一步检测HIV-1抗体直接在临床样本。
Diagnosis of infectious disease in patients, including human immunodeficiency virus (HIV) infection, can be achieved through the detection of specific antibodies produced by the immune system. We have previously shown that macromolecules such as antibodies can be efficiently detected in complex biological samples by sterically inhibiting the hybridization of conjugated complementary DNA strands to electrode-bound DNA strands. Here, we report a peptide-mediated electrochemical steric hindrance hybridization assay, PeSHHA, specially for the detection of antibodies against the gp41 protein of HIV-1. We show that the sensitivity of this PeSHHA can be significantly enhanced using nanostructured electrodes and demonstrate the rapid, one-step detection of HIV-1 antibodies directly in clinical samples.