Localization Microscopy Reveals Expression-Dependent Parameters of Chromatin Nanostructure
Localization Microscopy Reveals Expression-Dependent Parameters of Chromatin Nanostructure
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DOI：
10.1016/j.bpj.2010.05.043
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发表时间：
2010-09-08
影响因子：
3.4
通讯作者：
Cremer, Christoph
中科院分区：
文献类型：
--
作者：
Bohn, Manfred;Diesinger, Philipp;Cremer, Christoph
提出了二维高分辨率定位光学显微镜和统计方法的组合方法来推断核纳米尺度的结构特征和密度波动。人类成纤维细胞和 HeLa 细胞都在 100 nm 以下的尺度上发现了核纳米结构的标志。从细胞内的组蛋白密度波动中提取机械测量作为定量工具，以获得几微米尺度的结构波动。结果表明，同一核蛋白类型的不同表达机制导致纳米尺度上显着不同的模式，以及检测到的染色质可压缩性的显着差异。观察到的波动，包括动态循环的实验证据，与最近提出的染色质模型一致。
A combined approach of 2D high-resolution localization light microscopy and statistical methods is presented to infer structural features and density fluctuations at the nuclear nanoscale. Hallmarks of nuclear nanostructure are found on the scale below 100 nm for both human fibroblast and HeLa cells. Mechanical measures were extracted as a quantitative tool from the histone density fluctuations inside the cell to obtain structural fluctuations on the scale of several micrometers. Results show that different mechanisms of expression of the same nuclear protein type lead to significantly different patterns on the nanoscale and to pronounced differences in the detected compressibility of chromatin. The observed fluctuations, including the experimental evidence for dynamic looping, are consistent with a recently proposed chromatin model.