In vivo models of proliferative vitreoretinopathy
In vivo models of proliferative vitreoretinopathy
复制标题
DOI：
10.1038/nprot.2007.4
复制
发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Hinton, David R.
中科院分区：
文献类型：
--
作者：
Agrawal, Rajat N.;He, Shikun;Hinton, David R.
我们概述了目前的体外和体内实验性增生性玻璃体视网膜病变（PVR）模型，并提供了我们的标准化体内PVR模型的详细协议。PVR是孔源性视网膜脱离手术失败的主要原因。这种多因素疾病的发病机制仍不完全清楚。PVR的实验模型帮助我们以受控的方式了解在疾病过程的发病机制中发挥作用的因素，并允许对新的治疗干预进行可重复的临床前评估。我们详细描述了一种细胞注射模型，该模型使用同源视网膜色素上皮（RPE）细胞培养物在2-8周的时间内诱导PVR。
We outline current in vitro and in vivo models for experimental proliferative vitreoretinopathy (PVR) and provide a detailed protocol of our standardized in vivo PVR model. PVR is the leading cause of failed surgical procedures for the correction of rhegmatogenous retinal detachment. The pathogenesis of this multifactorial condition is still not completely understood. Experimental models for PVR help us understand the factors that play a role in the pathogenesis of the disease process in a controlled manner and allow for reproducible preclinical assessment of novel therapeutic interventions. We describe a cell injection model in detail that uses homologous retinal pigment epithelial (RPE) cell cultures to induce PVR over a 2-8 week period.