A LIGHT AND ELECTRON-MICROSCOPIC ANTEROGRADE WGA-HRP TRACING STUDY ON THE SENSORY INNERVATION OF JUNCTIONAL AND SULCULAR EPITHELIUM IN THE RAT MOLAR
A LIGHT AND ELECTRON-MICROSCOPIC ANTEROGRADE WGA-HRP TRACING STUDY ON THE SENSORY INNERVATION OF JUNCTIONAL AND SULCULAR EPITHELIUM IN THE RAT MOLAR
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DOI：
10.1177/00220345920710011001
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发表时间：
1992-01-01
影响因子：
7.6
通讯作者：
TANAKA, T
中科院分区：
文献类型：
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作者：
KONDO, T;AYASAKA, N;TANAKA, T
利用小麦胚芽凝集素-辣根过氧化物酶从三叉神经节顺行转运的方法，研究了连接上皮和口腔上皮的感觉神经支配。光镜下可见标记的上皮内神经纤维在连接上皮顶端三分之二处形成密集的神经丛，部分纤维位于牙釉质间隙附近。偶有纤维冠状延伸至沟底。通过电子显微镜，标记的上皮内轴突终末或静脉曲张被证明与连接上皮细胞和中性粒细胞密切接触。这些静脉曲张偶尔被上皮细胞或中性粒细胞的细胞质突起包围，经常含有大颗粒和小透明囊泡。与连接上皮相比，口腔管状上皮的神经支配较少，除了在口腔管状上皮和连接上皮之间的过渡区域，发现有标记的上皮内纤维密集的神经支配。这些纤维一直延伸到棘层。电镜显示上皮细胞间充满线粒体的静脉曲张。本研究显示了感觉神经在口腔交界上皮和口腔沟状上皮的分布和超微结构的差异，进一步提供了形态学证据，表明交界上皮内的感觉神经不仅与上皮细胞接触，还与中性粒细胞接触。
The sensory innervation of junctional and oral sulcular epithelium was investigated by use of anterograde transport of wheat-germ agglutinin-horseradish peroxidase from the trigeminal ganglion. By light microscopy, labeled intra-epithelial nerve fibers were observed forming a dense plexus in the apical two-thirds of the junctional epithelium, with some fibers located near the enamel space. Occasional fibers extended coronally to the sulcus bottom. By electron microscopy, labeled intra-epithelial axon terminals or varicosities were demonstrated to be in close contact with both junctional epithelial cells and neutrophils. These varicosities, which were occasionally surrounded by the cytoplasmic processes of epithelial cells or neutrophils, frequently contained large granular and small clear vesicles. In contrast to the junctional epithelium, the oral sulcular epithelium was sparsely innervated, except for the transition region between the oral sulcular epithelium and the junctional epithelium, where a dense innervation by labeled intraepithelial fibers was found. These fibers extended as far as the stratum spinosum. Electron microscopy revealed mitochondria-filled profiles of varicosities between epithelial cells. This study shows differences in the distribution and ultrastructure of sensory nerves between the junctional and oral sulcular epithelia, and further provides morphological evidence that sensory nerves in the junctional epithelium come into contact not only with epithelial cells but also with neutrophils.