Programmable on-chip DNA compartments as artificial cells
Programmable on-chip DNA compartments as artificial cells
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DOI：
10.1126/science.1255550
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发表时间：
2014-08-15
期刊：
影响因子：
56.9
通讯作者：
Bar-Ziv, Roy H.
中科院分区：
文献类型：
--
作者：
Karzbrun, Eyal;Tayar, Alexandra M.;Bar-Ziv, Roy H.
组装能够执行合成DNA程序的人造细胞一直是基础研究和生物技术的一个重要目标。我们将二维 DNA 隔室组装在硅中，作为能够新陈代谢、可编程蛋白质合成和通信的人造细胞。新陈代谢是通过营养物质和产物通过细毛细管持续扩散来维持的，将 DNA 区室中的蛋白质合成与环境连接起来。我们在胚胎规模的一系列相互连接的区室中编程了蛋白质表达周期、自动调节的蛋白质水平和信号表达梯度（相当于形态发生素）。 DNA区室中的基因表达揭示了一个由几何形状控制的丰富的动态系统，为研究活细胞外的生物网络提供了一种手段。
The assembly of artificial cells capable of executing synthetic DNA programs has been an important goal for basic research and biotechnology. We assembled two-dimensional DNA compartments fabricated in silicon as artificial cells capable of metabolism, programmable protein synthesis, and communication. Metabolism is maintained by continuous diffusion of nutrients and products through a thin capillary, connecting protein synthesis in the DNA compartment with the environment. We programmed protein expression cycles, autoregulated protein levels, and a signaling expression gradient, equivalent to a morphogen, in an array of interconnected compartments at the scale of an embryo. Gene expression in the DNA compartment reveals a rich, dynamic system that is controlled by geometry, offering a means for studying biological networks outside a living cell.