START lipid/sterol-binding domains are amplified in plants and are predominantly associated with homeodomain transcription factors.
START lipid/sterol-binding domains are amplified in plants and are predominantly associated with homeodomain transcription factors.
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DOI：
10.1186/gb-2004-5-6-r41
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Mayer KF
中科院分区：
文献类型：
--
作者：
Schrick K;Nguyen D;Karlowski WM;Mayer KF
一项含有脂/固醇结合的星形相关脂转移(Start)结构域的蛋白质的研究表明，它们在植物中被扩增，并且主要在同源结构域(HD)转录因子中发现。在动物体内，类固醇激素通过与核受体结合来调节基因表达。植物缺乏核受体基因，但来自拟南芥的遗传证据表明，类脂/甾醇的发育作用类似于动物。与核受体不同，脂/固醇结合的星形相关脂转移(Start)蛋白结构域是保守的，这使得它们可能参与动物和植物的脂/固醇信号转导。我们从拟南芥、水稻、动物、原生生物和细菌的基因组中调查了可能的起始域。起始结构域在植物中比在动物中更常见，在植物中主要在同源结构域(HD)转录因子中发现。最大的HD-START蛋白亚家族的特征是HD氨基末端是植物特有的带有内环的亮氨酸拉链，而在一个较小的亚家族中，HD先于经典的亮氨酸拉链。植物HD-Start蛋白的起始域与动物的起始域没有密切的关系，这意味着侧枝进化以适应生物体特有的脂类/甾醇。利用哺乳动物START蛋白的晶体结构，我们证明了哺乳动物磷脂酰胆碱转移蛋白(PCTP)的START结构域在植物中的结构保守，与脂类运输和代谢中的共同作用一致。我们还描述了来自细菌和单细胞原生生物的假定的起始域蛋白。植物中的大多数起始结构域属于一类新的推测的脂/固醇结合转录因子，HD-START家族，它在植物界中是保守的。HD-Start蛋白仅限于植物中，这表明了一种脂/固醇配体可以直接调控植物转录的机制。
A survey of proteins containing lipid/sterol-binding StAR-related lipid transfer (START) domains shows that they are amplified in plants and are primarily found within homeodomain (HD) transcription factors. In animals, steroid hormones regulate gene expression by binding to nuclear receptors. Plants lack genes for nuclear receptors, yet genetic evidence from Arabidopsis suggests developmental roles for lipids/sterols analogous to those in animals. In contrast to nuclear receptors, the lipid/sterol-binding StAR-related lipid transfer (START) protein domains are conserved, making them candidates for involvement in both animal and plant lipid/sterol signal transduction. We surveyed putative START domains from the genomes of Arabidopsis, rice, animals, protists and bacteria. START domains are more common in plants than in animals and in plants are primarily found within homeodomain (HD) transcription factors. The largest subfamily of HD-START proteins is characterized by an HD amino-terminal to a plant-specific leucine zipper with an internal loop, whereas in a smaller subfamily the HD precedes a classic leucine zipper. The START domains in plant HD-START proteins are not closely related to those of animals, implying collateral evolution to accommodate organism-specific lipids/sterols. Using crystal structures of mammalian START proteins, we show structural conservation of the mammalian phosphatidylcholine transfer protein (PCTP) START domain in plants, consistent with a common role in lipid transport and metabolism. We also describe putative START-domain proteins from bacteria and unicellular protists. The majority of START domains in plants belong to a novel class of putative lipid/sterol-binding transcription factors, the HD-START family, which is conserved across the plant kingdom. HD-START proteins are confined to plants, suggesting a mechanism by which lipid/sterol ligands can directly modulate transcription in plants.
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影响因子：
14.9
作者：
Karlowski, WM;Schoof, H;Mayer, KFX
通讯作者：
Mayer, KFX
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
7.4
作者：
Baima, S;Possenti, M;Morelli, G
通讯作者：
Morelli, G
影响因子：
4.8
作者：
Kallen, CB;Billheimer, JT;Strauss, JF
通讯作者：
Strauss, JF
影响因子：
2.9
作者：
Choinowski, T;Hauser, H;Piontek, K
通讯作者：
Piontek, K