Enhancing In Vitro Bioactivity of Melt‐Derived 45S5 Bioglass® by Comminution in a Stirred Media Mill
Enhancing In Vitro Bioactivity of Melt‐Derived 45S5 Bioglass® by Comminution in a Stirred Media Mill
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DOI：
10.1111/jace.12615
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发表时间：
2014
影响因子：
3.9
通讯作者：
Stefan Romeis;A. Hoppe;C. Eisermann;N. Schneider;A. Boccaccini;Jochen Schmidt;W. Peukert
中科院分区：
文献类型：
--
作者：
Stefan Romeis;A. Hoppe;C. Eisermann;N. Schneider;A. Boccaccini;Jochen Schmidt;W. Peukert
45 S5 Bioglass®（45 S5 BG）是一种常用的A型生物活性材料，能够与骨和软组织形成固有的结合。目前，所应用的熔融衍生的生物活性玻璃粉末（BG）表现出几微米至几百微米之间的粒度。最近的研究纳米尺寸的生物活性玻璃（nBGs），由自下而上的方法，如溶胶-凝胶加工或火焰喷雾热解，生产表明其巨大的潜力，为几个生物医学应用。在这项研究中，自上而下的处理从散装45 S5 BG通过湿粉碎在搅拌介质磨的可行性进行了研究。通过在模拟体液中的体外羟基碳酸磷灰石（HCAp）形成来评估产品，HCAp是生物活性行为的标志物。该研究揭示了所用溶剂对成功的自上而下加工的最重要影响：与原材料相比，在水中加工的粉末失去了生物活性，保留在乙醇中粉碎，并增加了使用醇正丁醇，正戊醇和正己醇加工的粉末。还发现，只有对于后一种溶剂，玻璃的化学成分在粉碎过程中才能保持不变。获得比表面积为~25-30 m2/g的片状、略微多孔的颗粒。因此，所提出的粉碎方法提供了一种方便的技术来处理具有增强的生物活性的45 S5 BG。
45S5 Bioglass® (45S5 BG) is a frequently applied Type A bioactive material, capable of forming an inherent bond to bone and soft tissue. Currently, applied melt-derived bioactive glass powders (BG) exhibit particle sizes between a few to several hundred micrometers. Recent studies on nanometer-sized bioactive glasses (nBGs), produced by bottom-up methods like sol–gel processing or flame spray pyrolysis, have indicated their great potential for several biomedical applications. In this study, the feasibility of top-down processing starting from bulk 45S5 BG by wet comminution in a stirred media mill was investigated. The products were assessed by in vitro hydroxycarbonate apatite (HCAp) formation in simulated body fluid, which is a marker for bioactive behavior. The study reveals the paramount influence of the used solvent for a successful top-down processing: In comparison with the as-received material bioactivity is lost for powders processed in water, preserved for comminution in ethanol and increased for powders processed using the alcohols n-butanol, n-pentanol, and n-hexanol. It was also found that only for the latter solvents, the chemical composition of the glass is maintained during comminution. Flake-like, slightly porous particles with specific surface areas of ~25–30 m2/g are obtained. Thus, the presented comminution approach offers a convenient technique to process 45S5 BG with enhanced bioactivity.