A quantitative morphometrical study of neuron degeneration in the substantia nigra in Parkinson's disease
A quantitative morphometrical study of neuron degeneration in the substantia nigra in Parkinson's disease
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DOI：
10.1016/0022-510x(96)00069-x
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发表时间：
1996-09-01
影响因子：
4.4
通讯作者：
Rinne, UK
中科院分区：
文献类型：
--
作者：
Ma, SY;Rinne, JO;Rinne, UK
我们研究了黑质（SN）的色素神经元，从8个控制和20例帕金森病（PD）患者使用计算机形态计量学方法。根据神经元地形图，在SN中概述了几个解剖区域。在这些亚区的面积，周长，直径的细胞体和细胞数量进行了测量和计数在对照组和PD患者。在第三脑神经从脑干的出口水平进行测量。在PD患者中，当考虑整个SN时，色素沉着细胞体的平均面积、平均周长和直径分别比对照平均值显著减少35%、20%和21%。在区域上，与对照组相比，内侧腹侧部（VM）、内侧背侧部（DM）、外侧腹侧部（VL）、外侧背侧部（DL）和外侧部（PL）的色素神经元面积显著减少33-41%。在这些亚区中，PD患者的色素神经元周长和直径均较对照组平均值显著降低19- 26%和19- 25%。这种细胞大小的减少表明，在PD患者中，SN中剩余的色素神经元处于变性和萎缩的过程中。在PD患者中，整个SN中的色素沉着神经元的数量比对照值减少约76%。然而，评价细胞大小对切片中细胞表观数量的影响表明，在PD患者中，色素神经元的真实损失的影响远比其大小减少的影响更显著。
We studied the pigmented neurons of the substantia nigra (SN) from 8 controls and 20 patients with Parkinson's disease (PD) using a computerized morphometric methodology. On the basis of neuronal topography, several anatomic regions were outlined in the SN. In these subregions the area, perimeter, diameter of the cell bodies and cell numbers were measured and were counted in the controls and PD patients. The measurements were made at the level of the exit of the third cranial nerve from the brain stem. In PD patients, when the whole SN was considered, the mean area, mean perimeter and diameter of the pigmented cell bodies were significantly reduced by 35%, 20% and 21% respectively from the control mean values. Regionally, the pigmented neuron area in the medial ventral part (VM), medial dorsal part (DM), lateral ventral part (VL), lateral dorsal part (DL) and pars lateralis part (PL) showed a significant reduction of 33-41% as compared to controls. In these subregions, a significant decrease in PD patients from the control mean values was seen both in the pigmented neuron perimeter, by 19-26%, and the diameter by 19-25%. This decrease in cell size suggests that, in PD patients,the remaining pigmented neurons in the SN are in a process of degeneration and atrophy. In PD patients the number of pigmented neurons in the whole SN decreased about 76% from control values. Evaluation of the influence of cell size on the apparent quantity of cells in sections indicates, however, that in PD patients the impact of true loss of pigmented neurons is far more dramatic than the impact of their decrease in size.