Mechanical force-induced midpalatal suture remodeling in mice
Mechanical force-induced midpalatal suture remodeling in mice
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DOI：
10.1016/j.bone.2007.01.019
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发表时间：
2007-06-01
期刊：
影响因子：
4.1
通讯作者：
Olsen, Bjorn R.
中科院分区：
文献类型：
--
作者：
Hou, Bo;Fukai, Naomi;Olsen, Bjorn R.
机械应力是调节骨骼重塑的重要表观遗传因素，并且力的施加可导致骨和软骨的重塑。软骨细胞、成骨细胞和破骨细胞都参与了这一重塑过程，并相互作用。为了研究细胞对机械刺激的反应，在一个可以遗传操纵的系统中，我们在体内使用小鼠腭中缝扩张。通过以0.56 N的初始力打开环，使六周龄雄性C57 BL/6小鼠经受腭缝扩张1、3、5、7、14或28天的时间。扩张缝线中的骨膜细胞增殖增加，对照组中Ki 67阳性细胞占缝线总细胞的1.8 +/- 0.1%至4.5 +/- 0.4%，实验/扩张组中Ki 67阳性细胞占缝线总细胞的12.0 +/- 2.6%至19.9 +/- 1.2%（P
Mechanical stress is an important epigenetic factor for regulating skeletal remodeling, and application of force can lead to remodeling of both bone and cartilage. Chondrocytes, osteoblasts and osteoclasts all participate and interact with each other in this remodeling process. To study cellular responses to mechanical stirnuli in a system that can be genetically manipulated, we used mouse midpalatal suture expansion in vivo. Six-week-old male C57BL/6 mice were subjected to palatal suture expansion by opening loops with an initial force of 0.56 N for the periods of 1, 3, 5, 7, 14 or 28 days. Periosteal cells in expanding sutures showed increased proliferation, with Ki67-positive cells representing 1.8 +/- 0.1% to 4.5 +/- 0.4% of total suture cells in control groups and 12.0 +/- 2.6% to 19.9 +/- 1.2% in experimental/expansion groups (P