An oxygen insensitive microsensor for nitrous oxide
An oxygen insensitive microsensor for nitrous oxide
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DOI：
10.1016/s0925-4005(01)00924-8
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发表时间：
2001-12-15
影响因子：
8.4
通讯作者：
Revsbech, NP
中科院分区：
文献类型：
--
作者：
Andersen, K;Kjær, T;Revsbech, NP
提出了一种对O-2不敏感、快速响应的微型N2 O传感器的研制方法。通过在对O-2和N2 O都敏感的电化学微传感器的尖端前面附加具有抗坏血酸的碱性溶液的毛细管来获得O-2干扰的消除。当在空气饱和溶液（O-2分压21 kPa）中测量时，pH 12的2 M抗坏血酸盐的0.1 min层能够除去所有O-2，并且没有观察到抗坏血酸盐对N2 O的还原。该传感器对N_2O的响应在0 ~ 1.2mM范围内呈线性，检测限小于1 μ M。详细描述的传感器的尖端直径为50-80 μ m，但是可以构造小得多的传感器。90%的响应时间约为40-50 s。二氧化碳的干扰被证明是可以忽略不计的环境监测方面，乙炔的灵敏度。其通常用于在反硝化研究中抑制一氧化二氮还原酶，可以保持足够低的浓度用于实验工作。(C)2001 Elsevier Science B. V.保留所有权利。
A method to create an O-2 insensitive and fast responding microsensor for N2O is presented. The elimination of O-2 interference was obtained by attaching a capillary with an alkaline solution of ascorbate in front of the tip of an electrochemical microsensor that was sensitive to both O-2 and N2O. A 0.1 min layer of 2 M ascorbate at pH 12 was able to remove all O-2 when measuring in air-saturated solutions (O-2 partial pressure 21 kPa), and no reduction of N2O by the ascorbate was observed. The response of the sensor to N2O was linear from 0 to 1.2 mM, and the detection limit was less than 1 muM. The tip diameters of the sensors described in detail were 50-80 mum, but much smaller sensors can be constructed. The 90% response times were about 40-50 s. Interference of carbon dioxide was shown to be negligible with respect to environmental monitoring, and the sensitivity to acetylene. which is commonly used to inhibit nitrous oxide reductase in denitrification studies, could be kept sufficiently low for experimental work. (C) 2001 Elsevier Science B.V. All rights reserved.