Production of 2-hydroxy 4-methoxy benzaldehyde using root cultures of Hemidesmus indicus
Production of 2-hydroxy 4-methoxy benzaldehyde using root cultures of Hemidesmus indicus
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DOI：
10.1023/a:1005354003727
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发表时间：
1998-07-01
影响因子：
2.7
通讯作者：
Pushpangadan, P
中科院分区：
文献类型：
--
作者：
Sreekumar, S;Seeni, S;Pushpangadan, P
在添加了2 mg吲哚-3-丁酸l（-1）的半浓度Murashige和Skoog培养基中黑暗培养的印度半鞭草（Hemidesmusindicus）植株的节段外植体在10天内产生10- 1 - 2个根（1- 2cm），且产生的愈伤组织最少。在Gamborg等人的培养基中培养类似于20 mg干重的根，该培养基补充有2 mg IBA 1（-1）、蔗糖（4%w/v）、pH（5.6）并在黑暗中搅拌（70 rpm），30天后在根中产生550 mg根干重/烧瓶和0.18%的2-羟基4-甲氧基苯甲醛。在21个月的时间内，根的正常形态、一致的生物量和产物产量的维持表明培养物的稳定性。
Nodal explants of Hemidesmus indicus plants cultured in half strength Murashige and Skoog medium fortified with 2 mg indole-3-butyric acid l(-1) in the dark produced 10-12 roots (1-2 cm) with minimal callusing in 10 days. Roots, similar to 20 mg dry wt, cultured in Gamborg et al. medium supplemented with 2 mg IBA l(-1), sucrose (4% w/v), pH (5.6) and agitation (70 rpm) in the dark yielded 550 mg root dry wt/flask and 0.18% 2-hydroxy 4-methoxy benzaldehyde in the roots after 30 days. The maintenance of normal morphology of the roots, consistent biomass and product yields over a 21-month period indicated the stability of the cultures.