Long-term surveillance of H7 influenza viruses in American wild aquatic birds: are the H7N3 influenza viruses in wild birds the precursors of highly pathogenic strains in domestic poultry?
Long-term surveillance of H7 influenza viruses in American wild aquatic birds: are the H7N3 influenza viruses in wild birds the precursors of highly pathogenic strains in domestic poultry?
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DOI：
10.1038/emi.2015.35
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发表时间：
2015-06-17
影响因子：
13.2
通讯作者：
Webster RG
中科院分区：
文献类型：
--
作者：
Krauss S;Stucker KM;Schobel SA;Danner A;Friedman K;Knowles JP;Kayali G;Niles LJ;Dey AD;Raven G;Pryor P;Lin X;Das SR;Stockwell TB;Wentworth DE;Webster RG
甲型流感病毒（IAV）在家禽物种中的出现以及向哺乳动物的相关传播是不可预测的。在美洲，H7 IAV特别令人担忧，2002年至2012年期间，北美和南美的家禽中发生了四次高致病性（HP）H7 N3的独立爆发，偶尔会蔓延到人类。在这里，我们使用1985年至2012年在美国特拉华州湾的北美滨鸟和加拿大阿尔伯塔的鸭子中进行的长期IAV监测，从1976年到2012年，以确定哪些血凝素（HA）-神经氨酸酶（NA）组合在雁形目（鸭）和？（水鸟）的H7流行和传播高峰与家禽和人类出现H7 IAV之间是否存在一致性。全基因组测序支持系统发育和基因组星座分析，以确定是否出现在特定的内部基因片段序列的背景下的HP IAV。野生鸟类H7 N3流感病毒和美洲HP H7 N3爆发的全基因组序列的系统发育分析表明，每次HP爆发都是一个独立的出现事件，低致病性（LP）禽流感前体最有可能来自鸭子。不同的多碱裂解位点在四个HP爆发支持独立的起源。在95%核苷酸同源性水平上，野鸭HA、PB 1和M基因序列与禽流感和人类流感病毒的基因序列聚在一起。基因组星座分析强烈表明，基因片段/病毒流从野生鸟类到家禽。
The emergence of influenza A virus (IAV) in domestic avian species and associated transmissions to mammals is unpredictable. In the Americas, the H7 IAVs are of particular concern, and there have been four separate outbreaks of highly pathogenic (HP) H7N3 in domestic poultry in North and South America between 2002 and 2012, with occasional spillover into humans. Here, we use long-term IAV surveillance in North American shorebirds at Delaware Bay, USA, from 1985 to 2012 and in ducks in Alberta, Canada, from 1976 to 2012 to determine which hemagglutinin (HA)–neuraminidase (NA) combinations predominated in Anseriformes (ducks) and Charadriiformes (shorebirds) and whether there is concordance between peaks of H7 prevalence and transmission in wild aquatic birds and the emergence of H7 IAVs in poultry and humans. Whole-genome sequencing supported phylogenetic and genomic constellation analyses to determine whether HP IAVs emerge in the context of specific internal gene segment sequences. Phylogenetic analysis of whole-genome sequences of the H7N3 influenza viruses from wild birds and HP H7N3 outbreaks in the Americas indicate that each HP outbreak was an independent emergence event and that the low pathogenic (LP) avian influenza precursors were most likely from dabbling ducks. The different polybasic cleavage sites in the four HP outbreaks support independent origins. At the 95% nucleotide percent identity-level phylogenetic analysis showed that the wild duck HA, PB1, and M sequences clustered with the poultry and human outbreak sequences. The genomic constellation analysis strongly suggests that gene segments/virus flow from wild birds to domestic poultry.