The effects of alginate lyase treatment on structural characteristics and anti-photoaging activity of alginate from Sargassum fusiforme
The effects of alginate lyase treatment on structural characteristics and anti-photoaging activity of alginate from Sargassum fusiforme
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DOI：
10.1002/fft2.218
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发表时间：
2023-02-24
期刊：
影响因子：
9.9
通讯作者：
Hileuskaya, Kseniya
中科院分区：
文献类型：
--
作者：
Chang, Shiyuan;Hu, Jinhong;Hileuskaya, Kseniya
从马尾藻中制备的藻酸盐（命名为P1）在我们的前期研究中已被证明具有显著的抗光老化活性。本研究利用海藻酸盐裂解酶获得了不同分子量的P1水解产物，并对其结构特征和抗光老化活性进行了比较。结果表明，海藻酸解酶能快速降解P1的分子量，并对P1的化学成分有轻微的改变。傅里叶红外光谱分析表明，海藻酸解酶处理对P1的主要官能团没有影响。此外，P1对MMPs （MMP-1、MMP-3和MMP-9）和活性氧的抑制作用也在海藻酸解酶水解后得到了改善，尤其是样品A-30（水解30min）。甲基化和核磁共振结果表明，A-30的主链为->)- β - mana -(1 ->)- α - gula -(1 ->)- β - mana -(1 ->)- α - gula -(1 ->)- β - mana -(1 ->)- α - gula -(1 -> 4)- β - mana -(1 -> 3,4)- β - mana -(1 ->), α - fucp -(1 ->)端基位于侧链上-> 3,4)- β - mana -(1 ->)的O-3位。说明海藻酸解酶在水解30 min时，并没有改变P1的主链，但降低了P1的分子聚合或聚合度。本文首次报道了海藻酸盐水解物的抗光老化活性。研究结果将为提高毛刺的利用率和促进毛刺的应用提供有效的理论支持。
An alginate (named P1) prepared from Sargassum fusiforme has been proven to have significant anti-photoaging activity in our previous study. In this study, alginate lyase was used to obtain P1 hydrolysates with different molecular weights, and their structural characteristics and anti-photoaging activity were compared. Results showed that alginate lyases could rapidly degrade the molecular weight of P1 and slightly changed its chemical composition. Fourier transform infrared spectra indicated that alginate lyase treatment did not change the main functional groups of P1. Moreover, the inhibitory effects of P1 on MMPs (MMP-1, MMP-3 and MMP-9) and reactive oxygen species were also improved after hydrolyzed with alginate lyase, especially sample A-30 (hydrolysis for 30 min). Methylation and nuclear magnetic resonance results indicated that the main chain of A-30 was -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 -> 4)-beta-ManA-(1 -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 -> 4)-beta-ManA-(1 -> 3, 4)-beta-ManA-(1 ->, with the end group of alpha-Fucp-(1 -> attached to the O-3 position of -> 3, 4)-beta-ManA-(1 -> on the side chain. It indicated that alginate lyase did not change the backbone of P1 but decreased the molecular or degree of polymerization at 30 min of hydrolysis. This is the first report on the anti-photoaging activity of alginate hydrolysates. The results will provide effective theoretical support for improving the utilization and promoting the application of S. fusiforme.