Biomimetic polymer nanostructures by injection molding
Biomimetic polymer nanostructures by injection molding
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DOI：
10.1002/mame.200290037
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发表时间：
2003-01-31
影响因子：
3.9
通讯作者：
Larsen, NB
中科院分区：
文献类型：
--
作者：
Gadegaard, N;Mosler, S;Larsen, NB
动物体内自组装结构蛋白复合物的纳米尺度形貌被认为诱导有利的细胞反应。这种纳米结构生物复合物的一个重要实例是纤维状胶原，其具有周期性为69 nm和峰谷距离为4 - 6 nm的交叉条纹结构。将牛I型胶原在体外组装成纤维状结构并沉积在固体支持物上。他们的结构主题转移到镍复制品的小晶粒金属层的物理气相沉积，然后电镀。发现所得的倒置镍结构忠实地呈现生物原始的大部分微米和纳米尺度形貌。这种镍复制品被用作一系列不同热塑性聚合物的注塑模具。总注塑循环时间在30 - 45秒的范围内。研究的聚合物材料之一，聚乙烯，显示生物纳米形貌图案的复制差。然而，大多数聚合物显示出非常高的复制保真度，如它们复制小于5nm高度差的交叉条纹特征的能力所证明的。后一组材料包括聚（丙烯）、聚（甲基丙烯酸甲酯）、聚（L-乳酸）、聚己内酯以及环状和线性烯烃的共聚物（COC）。这项工作表明，目前的限制因素的注塑成型的纳米级形貌的热塑性聚合物在于模具的初始金属涂层的晶粒尺寸，而不是聚合物本身。
The nanometer scale topography of self-assembling structural protein complexes in animals is believed to induce favorable cell responses. An important example of such nanostructured biological complexes is fibrillar collagen that possesses a cross-striation structure with a periodicity of 69 nm and a peak-to-valley distance of 4-6 nm. Bovine collagen type I was assembled into fibrillar structures in vitro and sedimented onto solid supports. Their structural motif was transferred in a nickel replica by physical vapor deposition of a small-grained metal layer followed by galvanic plating. The resulting inverted nickel structure was found to faithfully present most of the micrometer and nanometer scale topography of the biological original. This nickel replica was used as a die for the injection molding of a range of different thermoplastic polymers. Total injection molding cycle times were in the range of 30-45 seconds. One of the polymer materials investigated, polyethylene, display poor replication of the biological nanotopographical motif. However, the majority of the polymers showed very high replication fidelity as witnessed by their ability to replicate the cross-striation features of less than 5 nm height difference. The latter group of materials includes poly(propylene), poly(methyl methacrylate), poly(L-lactic acid), polycaprolactone, and a copolymer of cyclic and linear olefins (COC). This work suggests that the current limiting factor for the injection molding of nanometer scale topography in thermoplastic polymers lies with the grain size of the initial metal coating of the mold rather than the polymers themselves.