COMPLIANCE OF HIPPOCAMPAL-NEURONS TO PATTERNED SUBSTRATE NETWORKS
COMPLIANCE OF HIPPOCAMPAL-NEURONS TO PATTERNED SUBSTRATE NETWORKS
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DOI：
10.1002/jnr.490300204
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发表时间：
1991-10-01
影响因子：
4.2
通讯作者：
BREWER, GJ
中科院分区：
文献类型：
--
作者：
COREY, JM;WHEELER, BC;BREWER, GJ
在体外，神经元的生长可以通过低密度的细胞培养和细胞与基质之间的差异性黏附来控制。原代培养的大鼠海马神经元在无血清培养中生长在涂有聚赖氨酸的玻璃片上，形成不同线宽(3、5和10 mU-m)、相交距离(80、120和160 mU-m)和结节(相交)直径(5、10和20 mU-m)的聚赖氨酸网格。SOMAE不仅强烈地偏爱未消融的聚赖氨酸区域，而且它们也迁移到未消融的多赖氨酸区域较高的局部区域。这些轨迹是节点，而不是狭窄的连接路径；与较小的节点相比，是较大的节点。当路径宽度为5微米、节点直径为20微米、路径长度为80微米时，向节点的最大迁移率为88%。每天的观察表明，粘附性较大的区域活跃迁移，这解释了依从性差异的原因。
Neuronal growth can be controlled in vitro by plating cells at low density and by differential adhesion between the cell and substrate. Primary cultures of rat hippocampal neurons were grown in serum-free culture on polylysine-coated glass coverslips patterned by selective laser ablation so as to leave grids of polylysine with varying linewidths (3, 5, and 10-mu-m), intersection distance (80, 120, and 160-mu-m), and nodal (intersection) diameter (5, 10, and 20-mu-m). Not only did somae strongly prefer the unablated polylysine areas, but they also migrated to loci where the local area of unablated polylysine was higher. These loci were the nodes, as opposed to the narrow connecting paths, and larger nodes, as compared with smaller nodes. Maximum migration to nodes of 88% occurred for a combination of 5-mu-m pathwidth, 20-mu-m node diameter, and 80-mu-m pathlength. Daily observations indicated active migration to larger adhesive areas, which explains the differential compliance.