1H NMR studies of bacterial class I cytochromes c
1H NMR studies of bacterial class I cytochromes c
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细菌 I 类细胞色素 c 的 1H NMR 研究
DOI：
10.1016/0162-0134(86)80100-3
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发表时间：
1986
影响因子：
3.9
通讯作者：
G. Moore
中科院分区：
文献类型：
--
作者：
G. Moore
本文研究了红杜鹃花色素550、细叶红杜鹃花色素553和Rps的核磁共振（NMR）光谱特性。capsulata（菌株S.L.和2.3.1）细胞色素2。将细胞色素C_（550）和C_（553）与假单胞菌细胞色素C_（551）的光谱进行了比较。它们之间的序列同源性被发现反映了相似的NMR光谱性质的共振的一些不变的基团。包装周围的血红素丙酸被发现是相似的，尽管取代的氨基酸与丙酸相互作用，虽然在这个区域之间的cytochromec 550和c553的构象差异似乎是可能的。两种细胞色素c 2具有不同的序列和氧化还原电位[Pettigrew等人，生物化学生物Biophys. Acta 503，509-523（1979）]，但两个氨基酸取代仅引起可能是靠近取代位点的侧链重排的微小构象变化。
Nuclear magnetic resonance (NMR) spectral properties ofRhodopseudomonas gelatinosacytochromec550,Rhodospirillum tenuecytochromec553, andRps. capsulata(strains S.L. and 2.3.1) cytochromec2are reported. Spectra of cytochromesC550andC553are compared with those of Pseudomonad cytochromec551. The sequence homology between them was found to be reflected by similar NMR spectral properties for resonances of some invariant groups. Packing around a heme propionate was found to be similar, despite substitutions of amino acids interacting with the propionate, although a conformational difference in this region between cytochromec550andc553appears likely. The two cytochromesc2have different sequences and redox potentials [Pettigrew et al.,Biochim. Biophys. Acta503, 509–523 (1979)], but the two amino acid substitutions only cause minor conformational changes that are probably rearrangements of side chains close to the substitution sites.