Nanorg Microbial Factories: Light-Driven Renewable Biochemical Synthesis Using Quantum Dot-Bacteria Nanobiohybrids
Nanorg Microbial Factories: Light-Driven Renewable Biochemical Synthesis Using Quantum Dot-Bacteria Nanobiohybrids
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DOI：
10.1021/jacs.9b02549
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发表时间：
2019-07-03
影响因子：
15
通讯作者：
Nagpal, Prashant
中科院分区：
文献类型：
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作者：
Ding, Yuchen;Bertram, John R.;Nagpal, Prashant
活细胞不会与纳米材料自然接触，尽管这种人造生物体可能具有前所未有的多功能特性，例如使用电磁刺激无线激活酶功能。在纳米生物杂交有机体（或纳米有机体）中实现这种界面需要（1）通过亲和结合和自组装的化学偶联，（2）人工材料的光电状态与细胞过程之间的能量偶联，以及（3）确保生物相容性的适当界面的设计。在这里，我们展示了七种不同的核壳量子点（QD），激发范围从紫外到近红外能量，与细菌中的靶向酶位点相结合。当被光照射时，这些量子点使用二氧化碳（CO2），水和氮气（来自空气）作为底物驱动不同生物燃料和化学品的可再生生产。这些量子点使用它们的富锌壳面用于与蛋白质的亲和连接。半胱氨酸两性配体能够通过细胞摄取，促进细胞存活。总之，这些纳米团催化光诱导的空气水CO2还原，其高转换数（TON）类似于10（6）-10（8）（每摩尔细胞的产物摩尔数）转化为生物燃料，如异丙醇（IPA）、2，3-丁二醇（BDO）、C-11-C-15甲基酮（MK）和氢（H-2）;和化学品，如甲酸（FA）、氨（NH3）、乙烯（C2 H4）和可降解生物塑料聚羟基丁酸酯（PHB）。因此，这些静息细胞作为由光驱动的纳米微生物工厂发挥作用。
Living cells do not interface naturally with nanoscale materials, although such artificial organisms can have unprecedented multifunctional properties, like wireless activation of enzyme function using electromagnetic stimuli. Realizing such interfacing in a nanobiohybrid organism (or nanorg) requires (1) chemical coupling via affinity binding and self-assembly, (2) the energetic coupling between optoelectronic states of artificial materials with the cellular process, and (3) the design of appropriate interfaces ensuring biocompatibility. Here we show that seven different core shell quantum dots (QDs), with excitations ranging from ultraviolet to near-infrared energies, couple with targeted enzyme sites in bacteria. When illuminated by light, these QDs drive the renewable production of different biofuels and chemicals using carbon-dioxide (CO2), water, and nitrogen (from air) as substrates. These QDs use their zinc-rich shell facets for affinity attachment to the proteins. Cysteine zwitterion ligands enable uptake through the cell, facilitating cell survival. Together, these nanorgs catalyze light-induced air water CO2 reduction with a high turnover number (TON) of similar to 10(6)-10(8) (mols of product per mol of cells) to biofuels like isopropanol (IPA), 2,3-butanediol (BDO), C-11-C-15 methyl ketones (MKs), and hydrogen (H-2); and chemicals such as formic acid (FA), ammonia (NH3), ethylene (C2H4), and degradable bioplastics polyhydroxybutyrate (PHB). Therefore, these resting cells function as nanomicrobial factories powered by light.