14-3-3 proteins in Lewy bodies in Parkinson disease and diffuse Lewy body disease brains
14-3-3 proteins in Lewy bodies in Parkinson disease and diffuse Lewy body disease brains
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DOI：
10.1093/jnen/61.3.245
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发表时间：
2002-03-01
影响因子：
3.2
通讯作者：
Budka, H
中科院分区：
文献类型：
--
作者：
Kawamoto, Y;Akiguchi, I;Budka, H
路易体的几个组成部分已被确定。但路易体形成的确切机制尚未确定。14-3-3蛋白家族参与许多信号转导途径，并与α-突触核蛋白相互作用，α-突触核蛋白是路易体的主要成分。阐明14-3-3蛋白在路易体相关神经退行性疾病中的作用。我们对5名老年对照受试者和10名帕金森病（PD）或弥漫性路易体病（DLBD）患者的脑中的14-3-3进行了免疫组织化学研究。在正常对照组中，14-3-3样免疫反应主要见于皮质和皮质下各区域的神经元胞体和突起。在PD和DLBD病例中，发现了类似的免疫染色模式，并且在严重受累区域的存活神经元中免疫反应性通常是幸免的。此外，经典和皮质路易体强烈的免疫标记和一些营养不良的神经突也免疫反应14-3-3。我们的研究结果表明，14-3-3蛋白可能与路易体的形成，并可能在PD和DLBD的发病机制中发挥重要作用。
Several components of Lewy bodies have been identified. but the precise mechanism responsible for the formation of Lewy bodies remains undetermined. The 14-3-3 protein family is involved in numerous signal transduction pathways and interacts with alpha-synuclein, which is a major constituent of Lewy bodies. To elucidate the role of 14-3-3 proteins in neuro-degenerative disorders associated with Lewy bodies. we performed immunohistochemical studies on 14-3-3 in brains from 5 elderly control subjects and from 10 patients with Parkinson disease (PD) or diffuse Lewy body disease (DLBD). In the normal controls, 14-3-3-like immunoreactivity, was mainly observed in the neuronal somata and processes in various cortical and subcortical regions. In the PD and DLBD cases, a similar immunostaining pattern was found and immunoreactivity was generally spared in the surviving neurons from the severely affected regions. In addition, both classical and cortical Lewy bodies were intensely immunolabeled and some dystrophic neurites were also immunoreactive for 14-3-3. Our results suggest that 14-3-3 proteins may be associated with Lewy body formation and may play an important role in the pathogenesis of PD and DLBD.