Identification of a steroidogenic neurohormone in female mosquitoes
Identification of a steroidogenic neurohormone in female mosquitoes
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DOI：
10.1074/jbc.273.7.3967
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发表时间：
1998-02-13
影响因子：
4.8
通讯作者：
Lea, AO
中科院分区：
文献类型：
--
作者：
Brown, MR;Graf, R;Lea, AO
在雌性蚊子埃及伊蚊中，神经激素从大脑中释放以响应血液餐，并刺激卵巢分泌蜕皮类固醇激素，其调节脂肪体中的蛋黄蛋白质合成。从头部提取物中分离出具有这种生物活性的神经肽，当比对时，这些肽的部分序列在氨基末端给出了31个残基的序列。该序列的寡核苷酸引物用于用聚合酶链式反应扩增与来自头部cDNA文库的克隆杂交的基因组DNA产物。cDNA编码149个残基的前激素原，其被加工成86个残基的肽，如从天然肽获得的质量值所示，具有预期的氨基末端序列。修饰后，推定的神经激素的cDNA在细菌系统中表达，并且纯化的肽在生物测定中具有高的比活性，从而证实它是类固醇生成性促性腺激素，这是第一个被确认的无脊椎动物。
In the female mosquito, Aedes aegypti, neurohormones are released from the brain in response to a blood meal and stimulate the ovaries to secrete ecdysteroid hormones, which modulate yolk protein synthesis in the fat body. Neuropeptides with this bioactivity were isolated from head extracts, and partial sequences from these peptides when aligned gave a 31-residue sequence at the amino terminus. Oligonucleotide primers for this sequence were used to amplify with the polymerase chain reaction a genomic DNA product that hybridized to a clone from a head cDNA library. The cDNA encodes a 149-residue preprohormone that is processed into an 86-residue peptide, as indicated by the mass value obtained from the native peptide, with the expected amino-terminal sequence, After modification, the cDNA for the putative neurohormone was expressed in a bacterial system, and the purified peptide had high specific activity in bioassays, thus confirming that it is a steroidogenic gonadotropin, the first to be identified for invertebrates.