Laminin α1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues
Laminin α1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues
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DOI：
10.1006/excr.2000.4883
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发表时间：
2000-06-15
影响因子：
3.7
通讯作者：
Ekblom, P
中科院分区：
文献类型：
--
作者：
Virtanen, I;Gullberg, D;Ekblom, P
产生了两种新型单克隆抗体，用于研究发育中和成人组织中层粘连蛋白 (Ln) α 1 链的表达。在胎儿和成人肾脏中，在最近端肾小管的基底膜 (BM) 中观察到明显的免疫反应性，但在远端肾小管或肾小球 BM 或血管基底膜中未观察到。代谢标记的培养人肾近端肾小管细胞的免疫沉淀显示 Ln α 1 链大量产生并沉积到细胞外基质，表明肾 Ln-1 的上皮起源。 JAR 绒毛膜癌细胞的定量细胞粘附实验表明，纯化的人 Ln-1 是细胞粘附的良好底物，与小鼠 Ln-1 相比，整合素受体对它的识别有所不同。在胎儿和成人睾丸中，免疫反应性仅局限于生精上皮的BM。在气道中，BM 限制反应仅见于处于假腺体发育阶段的胎儿出芽支气管（16-19 周）。在皮肤中，明显的免疫反应性仅限于发育中的毛芽的BM，但不存在于成人表皮或表皮附属物的上皮BM中。在其他成人组织中，在甲状腺、唾液腺和乳腺的骨髓以及子宫内膜和宫颈内膜的骨髓中发现了免疫反应性，但在子宫颈外或阴道的骨髓中没有发现免疫反应性。除食管粘膜下腺和十二指肠布伦纳腺的骨髓外，大多数消化道（包括消化道和胰腺）的骨髓中未发现免疫反应性。在胎儿标本中，肠和胃腺底部的 BM 呈阳性。血管基底层的 Ln α 1 链通常呈阴性，但胎儿和成人中枢神经系统样本除外，其中 Ln α 1 链的免疫反应性明显局限于毛细血管壁。结果表明，在中枢神经系统之外，Ln α 1 链在不同上皮组织的 BM 中显示出受限且发育调节的表达。 (C) 2000 年学术出版社。
Two novel monoclonal antibodies were raised and used to study the expression of laminin (Ln) alpha 1-chain in developing and adult human tissues. In both fetal and adult kidney, a distinct immunoreactivity was seen in basement membranes (BM) of most proximal tubules but not in the distal tubular or glomerular BM or in the basal laminae of blood vessels. Immunoprecipitation of metabolically labeled cultured human renal proximal tubular cells showed an abundant production and deposition of Ln alpha 1-chain to the extracellular matrix, suggestive of an epithelial origin of kidney Ln-1. Quantitative cell adhesion experiments with JAR choriocarcinoma cells showed that purified human Ln-1 is a good substrate for cell adhesion that it is differently recognized by integrin receptors when compared to mouse Ln-1. In fetal and adult testes immunoreactivity was solely confined to BM of the seminiferous epithelium. In the airways BM-confined reaction was only seen in fetal budding bronchial tubules (16-19 weeks) at the pseudoglandular stage of development. In the skin a distinct immunoreactivity was confined to BM of developing hair buds but not in epithelial BMs of adult epidermis or of epidermal appendages. In other adult tissues, immunoreactivity was found in BMs of thyroid, salivary, and mammary glands as well as in BMs of endometrium and endocervix, but not of ectocervix or vagina. No immunoreactivity was found in BMs of most of the digestive tract, including the fiver and pancreas, except for BMs of esophageal submucosal glands and duodenal Brunner's glands. In fetal specimens, BMs of the bottoms of the intestinal and gastric glands were positive. Basal laminae of blood vessels were generally negative for Ln alpha 1 chain with the exception of specimens of both fetal and adult central nervous system in which immunoreactivity for Ln alpha 1 chain was prominently confined to capillary walls. The results suggest that outside the central nervous system, Ln alpha 1 chain shows a restricted and developmentally regulated expression in BMs of distinct epithelial tissues. (C) 2000 Academic Press.