Massively parallel recording of unit and local field potentials with silicon-based electrodes
Massively parallel recording of unit and local field potentials with silicon-based electrodes
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DOI：
10.1152/jn.00116.2003
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发表时间：
2003-08-01
影响因子：
2.5
通讯作者：
Buzsáki, G
中科院分区：
文献类型：
--
作者：
Csicsvari, J;Henze, DA;Buzsáki, G
在行为动物中并行记录神经元活动是我们理解神经元表征和信息存储的先决条件。在这里，我们描述了微加工硅微电极阵列的单位和局部场记录的发展。具有96或64个记录位点的二维探针提供了单位和场活动的高密度记录，具有最小的组织移位或损伤。片上有源电路消除了运动和其他伪影，大大减轻了头盔的重量。记录尖端的精确几何形状允许估计所记录的神经元的空间位置和高分辨率估计细胞外电流源密度。同一神经元的索马和树突可同时记录到动作电位。硅技术是一种很有前途的方法，在基础研究和假体设备的神经元活动的高密度，高分辨率的采样。
Parallel recording of neuronal activity in the behaving animal is a prerequisite for our understanding of neuronal representation and storage of information. Here we describe the development of micro-machined silicon microelectrode arrays for unit and local field recordings. The two-dimensional probes with 96 or 64 recording sites provided high-density recording of unit and field activity with minimal tissue displacement or damage. The on-chip active circuit eliminated movement and other artifacts and greatly reduced the weight of the headgear. The precise geometry of the recording tips allowed for the estimation of the spatial location of the recorded neurons and for high-resolution estimation of extracellular current source density. Action potentials could be simultaneously recorded from the soma and dendrites of the same neurons. Silicon technology is a promising approach for high-density, high-resolution sampling of neuronal activity in both basic research and prosthetic devices.