Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B1 in agricultural product samples based on peroxidase-like spatial network structure.
Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B1 in agricultural product samples based on peroxidase-like spatial network structure.
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DOI：
10.1016/j.foodchem.2023.135856
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发表时间：
2023-03
期刊：
影响因子：
8.8
通讯作者：
Jingyu Kuang;J. Ju;Yongli Lu;Yitong Chen;Chang Liu;Dezhao Kong;Wei Shen;Haiwei Shi;Li L
中科院分区：
文献类型：
--
作者：
Jingyu Kuang;J. Ju;Yongli Lu;Yitong Chen;Chang Liu;Dezhao Kong;Wei Shen;Haiwei Shi;Li L
建立了一种基于过氧化物酶空间网络结构的高灵敏度黄曲霉毒素B1（AFB1）检测方法。将黄曲霉毒素B1的特异性抗体和抗原包被在组氨酸修饰的Fe 3 O4纳米酶上，形成捕获/检测探针。基于竞争/亲和效应，探针构建了空间网络结构，可通过磁三相单滴微萃取过程快速（8 s）分离。在此单滴微反应器中，利用该网络结构催化3，3 ′，5，5 ′-四甲基联苯胺氧化反应检测黄曲霉毒素B1。由于空间网络结构的强过氧化物酶样能力和微萃取的富集作用，信号被显著放大。因此，达到了低检测限（0.034 pg/mL）。该方法可消除真实的样品的基体效应，并通过农产品样品的分析验证了该方法的实用性。
In this work, a highly sensitive method for aflatoxin B1(AFB1) detection was developed based on a peroxidase-like spatial network structure. The specific antibody and antigen of AFB1were coated on a histidine-modified Fe3O4nanozyme to form the capture/detection probes. Based on the competition/affinity effect, the spatial network structure was constructed by the probes, which could be rapidly (8 s) separated by a magnetic three-phase single-drop microextraction process. In this single-drop microreactor, the network structure was applied to catalyze a colorimetric 3,3′,5,5′-tetramethylbenzidine oxidation reaction for AFB1detection. The signal was amplified significantly due to the strong peroxidase-like ability of the spatial network structure and the enrichment effect of the microextraction. Thus, a low detection limit (0.034 pg/mL) was achieved. The matrix effect of real sample can be eliminated by the extraction approach, and the practicability of this method was proved by agricultural product samples analysis.