Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome
Effect of Sleeve Gastrectomy on Kisspeptin Expression in the Hypothalamus of Rats with Polycystic Ovary Syndrome
复制标题
袖状胃切除术对多囊卵巢综合征大鼠下丘脑Kisspeptin表达的影响
DOI：
10.1002/oby.22795
复制
发表时间：
2020-04-29
期刊：
影响因子：
6.9
通讯作者：
Wen, Junping
中科院分区：
文献类型：
--
作者：
Wen, Lingying;Lin, Wei;Wen, Junping
目的探讨多囊卵巢综合征（PCOS）大鼠下丘脑Kissspeptin-1（Kiss 1）表达水平的变化及其在袖状胃切除术（SG）治疗后的变化。结果经SG治疗后，PCOS动物模型糖耐量降低、胰岛素敏感性降低、脂联素水平降低、动情周期紊乱、性激素水平升高等均恢复正常。此外，SG还能恢复PCOS大鼠弓状核Kiss 1 mRNA表达和Kiss 1阳性神经元的增加。有趣的是，虽然SG没有导致在食物饮食下施用DHEA的大鼠的体重显著减轻，结论SG后下丘脑Kiss 1表达水平恢复正常，提示Kiss 1可能在PCOS的发生发展中起重要作用，并可能与PCOS的发病机制有关。SG的改进。
Objectives The purpose of this study was to determine changes in the expression levels of kisspeptin-1 (Kiss1) in the hypothalamus during the development of polycystic ovary syndrome (PCOS) and after treatment with sleeve gastrectomy (SG).Methods This study used chronic dehydroepiandrosterone (DHEA) alone and DHEA plus a high-fat diet (HFD) to generate a PCOS rat model. Subsequently, SG was performed in the animals with PCOS and the effects on glucose tolerance, insulin sensitivity, sex hormones, estrous cyclicity, adiponectin, and Kiss1 expression in the hypothalamus were investigated.Results Impaired glucose tolerance, decreased insulin sensitivity, reduced adiponectin levels, disrupted estrous cyclicity, and elevated sex hormone levels associated with PCOS models were restored to normal following SG. In addition, SG was able to restore the increase in the expression of Kiss1 mRNA and Kiss1-positive neurons in the arcuate nucleus of rats with PCOS. Interestingly, although SG did not result in a significant loss of body weight in rats administered DHEA under a chow diet, it resulted in comparable metabolic improvements and Kiss1 expression in rats that had been administered DHEA along with an HFD.Conclusions The recovery of normal levels of Kiss1 expression in the hypothalamus after SG in this study suggests that Kiss1 might play an important role in the development of PCOS and its improvement by SG.